Expression of the mau gene cluster of Paracoccus denitrificans is controlled by MauR and a second transcription regulator
| Autor: | T. T. Huisman, A. H. Stouthamer, R. J. M. Van Spanning, C.O. Delorme, Y.-L. Chan, Willem Reijnders, N. Harms |
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| Přispěvatelé: | Molecular Cell Physiology, Chemistry and Biology, Molecular Microbiology, Systems Bioinformatics, AIMMS |
| Rok vydání: | 1997 |
| Předmět: |
Operon
Research Support Microbiology Promoter Regions Genetic Transcription (biology) Gene expression Gene cluster Escherichia coli Journal Article Transcriptional regulation Methylamine dehydrogenase Non-U.S. Gov't Promoter Regions Genetic Paracoccus denitrificans Oxidoreductases Acting on CH-NH Group Donors biology Research Support Non-U.S. Gov't Bacterial Promoter Gene Expression Regulation Bacterial biology.organism_classification Molecular biology Gene Expression Regulation Genes Genes Bacterial Multigene Family Trans-Activators |
| Zdroj: | Delorme, C, Huisman, T A, Reijnders, W N, Chan, Y L, Harms, N, Stouthamer, A H & van Spanning, R J 1997, ' Expression of the mau gene cluster of Paracoccus denitrificans is controlled by MauR and a second transcription regulator ', Microbiology, vol. 143 ( Pt 3), pp. 793-801 . https://doi.org/10.1099/00221287-143-3-793 Microbiology, 143 ( Pt 3), 793-801. Microbiology Society |
| ISSN: | 1465-2080 1350-0872 |
| DOI: | 10.1099/00221287-143-3-793 |
| Popis: | The mau gene cluster of Paracoccus denitrificans constitutes 11 genes (10 are located in the transcriptional order mauFBEDACJGMN; the 11th, mauR, is located upstream and divergently transcribed from these genes) that encode a functional methylamine-oxidizing electron transport branch. The mauR gene encodes a LysR-type transcriptional activator essential for induction of the mau operon. In this study, the characteristics of that process were established. By using lacZ transcriptional fusions integrated into the genome of P. denitrificans, it was found that the expression of the mauR gene during growth on methylamine and/or succinate was not autoregulated, but proceeded at a low and constant level. The mauF promoter activity was shown to be controlled by MauR and a second transcriptional regulator. This activity was very high during growth on methylamine, low on succinate plus methylamine, and absent on succinate alone. MauR was overexpressed in Escherichia coli by using a T7 RNA polymerase expression system. Gel shift assays indicated that MauR binds to a 403 bp DNA fragment spanning the mauR-mauF promoter region. It is concluded from these results that the expression of the structural mau genes is dependent on MauR and its inducer, methylamine, as well as on another transcription factor. Both activators are required for high-level transcription from the mauF promoter. It is hypothesized that the two activators act synergistically to activate transcription: the effects of the two activators are not additive and either one alone activates the mauF promoter rather weakly. |
| Databáze: | OpenAIRE |
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