Highly redundant neuropeptide volume co-transmission underlying episodic activation of the GnRH neuron dendron
| Autor: | Allan E. Herbison, Isaiah Cheong, H. James McQuillan, Sabine Hessler, Robert Porteous, Shel-Hwa Yeo, Michel K. Herde, Xinhuai Liu |
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| Přispěvatelé: | Herde, Michel K [0000-0002-2324-2083], Hessler, Sabine [0000-0002-4177-4825], Herbison, Allan E [0000-0002-9615-3022], Apollo - University of Cambridge Repository |
| Rok vydání: | 2021 |
| Předmět: |
Male
0301 basic medicine Mouse Dynorphin Gonadotropin-Releasing Hormone Mice chemistry.chemical_compound 0302 clinical medicine Kisspeptin Postsynaptic potential NKB Biology (General) Neurotransmitter Neurons GnRH Neuron 0303 health sciences General Neuroscience General Medicine 3. Good health medicine.anatomical_structure Medicine Female Neurokinin B hormones hormone substitutes and hormone antagonists Research Article pulse generator Dendrimers QH301-705.5 Science Neuropeptide GCaMP Biology General Biochemistry Genetics and Molecular Biology kisspeptin 03 medical and health sciences Glutamatergic Calcium imaging medicine Animals 030304 developmental biology General Immunology and Microbiology Neuropeptides 030104 developmental biology nervous system chemistry GnRH Neuron Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | eLife eLife, Vol 10 (2021) |
| DOI: | 10.17863/cam.64028 |
| Popis: | Funder: Health Research Council of New Zealand; FundRef: http://dx.doi.org/10.13039/501100001505 Funder: Wellcome Trust; FundRef: http://dx.doi.org/10.13039/100004440 The necessity and functional significance of neurotransmitter co-transmission remains unclear. The glutamatergic ‘KNDy’ neurons co-express kisspeptin, neurokinin B (NKB), and dynorphin and exhibit a highly stereotyped synchronized behavior that reads out to the gonadotropin-releasing hormone (GnRH) neuron dendrons to drive episodic hormone secretion. Using expansion microscopy, we show that KNDy neurons make abundant close, non-synaptic appositions with the GnRH neuron dendron. Electrophysiology and confocal GCaMP6 imaging demonstrated that, despite all three neuropeptides being released from KNDy terminals, only kisspeptin was able to activate the GnRH neuron dendron. Mice with a selective deletion of kisspeptin from KNDy neurons failed to exhibit pulsatile hormone secretion but maintained synchronized episodic KNDy neuron behavior that is thought to depend on recurrent NKB and dynorphin transmission. This indicates that KNDy neurons drive episodic hormone secretion through highly redundant neuropeptide co-transmission orchestrated by differential post-synaptic neuropeptide receptor expression at the GnRH neuron dendron and KNDy neuron. |
| Databáze: | OpenAIRE |
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