Phytochemical Profile, Free Radical Scavenging and Anti-Inflammatory Properties of Acalypha Indica Root Extract: Evidence from In Vitro and In Vivo Studies
| Autor: | Shanmugam Bhasha, Mallikarjuna Korivi, Ravi Sahukari, Shanmugam Kondeti Ramudu, Sathyavelu Reddy Kesireddy, Weibing Ye, Jyothi Punabaka, Venkata Subbaiah Ganjikunta |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Antioxidant
medicine.medical_treatment Glutathione reductase Pharmaceutical Science Organic chemistry Pharmacology Analytical Chemistry C-reactive protein Superoxide dismutase Lipid peroxidation chemistry.chemical_compound QD241-441 In vivo Drug Discovery medicine Gallic acid Physical and Theoretical Chemistry Indian Acalypha chemistry.chemical_classification biology Chemistry Glutathione peroxidase Ascorbic acid phytochemicals free radical scavengers Chemistry (miscellaneous) biology.protein Molecular Medicine edema |
| Zdroj: | Molecules, Vol 26, Iss 6251, p 6251 (2021) Molecules Volume 26 Issue 20 |
| ISSN: | 1420-3049 |
| Popis: | In our in vitro and in vivo studies, we used Acalypha indica root methanolic extract (AIRME), and investigated their free radical scavenging/antioxidant and anti-inflammatory properties. Primarily, phytochemical analysis showed rich content of phenols (70.92 mg of gallic acid/g) and flavonoids (16.01 mg of rutin/g) in AIRME. We then performed HR-LC-MS and GC-MS analyses, and identified 101 and 14 phytochemical compounds, respectively. Among them, ramipril glucuronide (1.563%), antimycin A (1.324%), swietenine (1.134%), quinone (1.152%), oxprenolol (1.118%), choline (0.847%), bumetanide (0.847%) and fenofibrate (0.711%) are the predominant phytomolecules. Evidence from in vitro studies revealed that AIRME scavenges DPPH and hydroxyl radicals in a concentration dependent manner (10–50 μg/mL). Similarly, hydrogen peroxide and lipid peroxidation were also remarkably inhibited by AIRME as concentration increases (20–100 μg/mL). In vitro antioxidant activity of AIRME was comparable to ascorbic acid treatment. For in vivo studies, carrageenan (1%, sub-plantar) was injected to rats to induce localized inflammation. Acute inflammation was represented by paw-edema, and significantly elevated (p < 0.05) WBC, platelets and C-reactive protein (CRP). However, AIRME pretreatment (150/300 mg/kg bodyweight) significantly (p < 0.05) decreased edema volume. This was accompanied by a significant (p < 0.05) reduction of WBC, platelets and CRP with both doses of AIRME. The decreased activities of superoxide dismutase, catalase, glutathione reductase and glutathione peroxidase in paw tissue were restored (p < 0.05 / p < 0.01) with AIRME in a dose-dependent manner. Furthermore, AIRME attenuated carrageenan-induced neutrophil infiltrations and vascular dilation in paw tissue. For the first time, our findings demonstrated the potent antioxidant and anti-inflammatory properties of AIRME, which could be considered to develop novel anti-inflammatory drugs. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|