Zerumbone, a Cyclic Sesquiterpene from Zingiber zerumbet Induces Apoptosis, Cell Cycle Arrest, and Antimigratory Effects in SW480 Colorectal Cancer Cells
| Autor: | K. B. Arun, B. P. Dhanya, Radhakrishnan Kokkuvayil Vasu, Mathew Dan, P. Nisha, Suresh Sini, Thomas Sithara |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cell cycle checkpoint Apoptosis Caspase 3 Caspase 8 03 medical and health sciences 0302 clinical medicine Cell Movement Zingiberaceae Cell Line Tumor Humans Caspase Cell Proliferation biology Plant Extracts Chemistry Cell growth Cell Cycle Checkpoints General Chemistry Cell cycle biology.organism_classification Molecular biology 030104 developmental biology Proto-Oncogene Proteins c-bcl-2 Zingiber zerumbet 030220 oncology & carcinogenesis biology.protein Colorectal Neoplasms General Agricultural and Biological Sciences Sesquiterpenes |
| Zdroj: | Journal of Agricultural and Food Chemistry. 66:602-612 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/acs.jafc.7b04472 |
| Popis: | Zerumbone isolated from the rhizomes of Zingiber zerumbet was investigated for the mechanisms by which it exhibits antiproliferative activity in colorectal cancer cells (SW480). The results indicated that the zerumbone suppressed cell growth and enhanced cell apoptosis. Exposure to zerumbone induced generation of reactive oxygen species, reduced the cellular antioxidant status, decreased mitochondrial membrane potential, and activated caspase 3, caspase 8, and caspase 9 (p < 0.001). It was also found that there was a decrease in the expression of Bcl 2 and elevation of Bax (p < 0.001) on exposure to zerumbone. Furthermore, treatment with 50, 75, and 100 μM zerumbone resulted in cell cycle arrest at the G2/M phase with a value of 17.2 ± 0.1, 19.63 ± 0.25, and 26.66 ± 0.25, respectively, and also distorted the microfilament network and effectively inhibited cellular migration. |
| Databáze: | OpenAIRE |
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