Screening and comparison of antioxidant activities of polysaccharides from Coriolus versicolor
| Autor: | Yanping Sun, Hai-Xue Kuang, Xiaowen Sun, Bing-You Yang, Zhibin Wang, Hongwei Zhang, Qiu-Hong Wang, Weiguo Zhu, Bin Li, Qing-Bo Zhang |
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| Rok vydání: | 2014 |
| Předmět: |
Trametes
chemistry.chemical_classification Chromatography Free Radicals Chemistry DPPH Rhamnose Monosaccharides Ion chromatography Drug Evaluation Preclinical Free Radical Scavengers General Medicine Uronic acid Glucuronic acid Polysaccharide Biochemistry High-performance liquid chromatography Molecular Weight Gel permeation chromatography chemistry.chemical_compound Polysaccharides Structural Biology Fruiting Bodies Fungal Molecular Biology |
| Zdroj: | International Journal of Biological Macromolecules. 69:12-19 |
| ISSN: | 0141-8130 |
| Popis: | Six polysaccharide fractions ( Coriolus versicolor polysaccharides: CVPS-1, CVPS-2, CVPS-3, CVPS-4, CVPS-5 and CVPS-6) were isolated and purified from the fruiting bodies of C. versicolor by ion exchange chromatography and gel chromatography. Their chemical and physical characteristics were determined by chemical methods, high performance liquid chromatography, and high-performance gel-permeation chromatography. Finally, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical assay, superoxide radical assay, and hydroxyl radical assay were carried out to test the antioxidant activities of CVPS in vitro . The results indicated that the six CVPS fractions were acidic heteropolysaccharides, composed of mannose, rhamnose, glucuronic acid, glucose and fructose with different ratios. The molecular weights of CVPS-1, CVPS-2, CVPS-3, CVPS-4, CVPS-5 and CVPS-6 were 1740, 1480, 568, 880, 1260 and 1840 kDa and the protein contents were 4.2%, 6.4%, 8.5%, 7.8%, 6.5% and 3.9%, respectively. Among the six fractions, CVPS with lower molecular weight, higher protein content and larger uronic acid amount, basically exhibited higher radical scavenging effects at the same concentration. Compared with other fractions, CVPS-3 exhibited the highest antioxidant activities. The effects of the molecular weight, protein content and uronic acid amount of the polysaccharides appeared to be significant on the improvement of the bioactivities. |
| Databáze: | OpenAIRE |
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