Alpha-synuclein from patient Lewy bodies exhibits distinct pathological activity that can be propagated in vitro
| Autor: | John Q. Trojanowski, Nicholas P. Marotta, Marshall G. Lougee, E. James Petersson, Emily S. Meymand, Jahan Ara, Virginia M.-Y. Lee, Bin Zhang, Norihito Uemura |
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| Rok vydání: | 2021 |
| Předmět: |
Somatic cell
Protein Conformation animal diseases Primary Cell Culture Fibril Pathology and Forensic Medicine law.invention Cellular and Molecular Neuroscience chemistry.chemical_compound Mice law Animals Humans RC346-429 Fluorescent Dyes Alpha-synuclein Synucleinopathies Brain Chemistry Mice Inbred C3H Chemistry Research Phenotype Immunohistochemistry In vitro Cell biology Mice Inbred C57BL nervous system Recombinant DNA alpha-Synuclein Female Lewy Bodies Neurology (clinical) Neurology. Diseases of the nervous system Autopsy Intracellular |
| Zdroj: | Acta Neuropathologica Communications Acta Neuropathologica Communications, Vol 9, Iss 1, Pp 1-18 (2021) |
| ISSN: | 2051-5960 |
| Popis: | Lewy bodies (LBs) are complex, intracellular inclusions that are common pathological features of many neurodegenerative diseases. They consist largely of aggregated forms of the protein alpha-Synuclein (α-Syn), which misfolds to give rise to beta-sheet rich amyloid fibrils. The aggregation of monomers into fibrils occurs readily in vitro and pre-formed fibrils (PFFs) generated from recombinant α-Syn monomers are the basis of many models of LB diseases. These α-Syn PFFs recapitulate many pathological phenotypes in both cultured cells and animal models including the formation of α-Syn rich, insoluble aggregates, neuron loss, and motor deficits. However, it is not clear how closely α-Syn PFFs recapitulate the biological behavior of LB aggregates isolated directly from patients. Direct interrogation of the cellular response to LB-derived α-Syn has thus far been limited. Here we demonstrate that α-Syn aggregates derived from LB disease patients induce pathology characterized by a prevalence of large somatic inclusions that is distinct from the primarily neuritic pathology induced by α-Syn PFFs in our cultured neuron model. Moreover, these LB-derived aggregates can be amplified in vitro using recombinant α-Syn to generate aggregates that maintain the unique, somatic pathological phenotype of the original material. Amplified LB aggregates also showed greater uptake in cultured neurons and greater pathological burden and more rapid pathological spread in injected mouse brains, compared to α-Syn PFFs. Our work indicates that LB-derived α-Syn from diseased brains represents a distinct conformation species with unique biological activities that has not been previously observed in fully recombinant α-Syn aggregates and demonstrate a new strategy for improving upon α-Syn PFF models of synucleinopathies using amplified LBs. Supplementary Information The online version contains supplementary material available at 10.1186/s40478-021-01288-2. |
| Databáze: | OpenAIRE |
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