T-2 metabolites in the excreta of broiler chickens administered 3H-labeled T-2 toxin
| Autor: | S P Swanson, Takumi Yoshizawa, Chester J. Mirocha |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Chromatography
Gas Chemical Phenomena medicine.disease_cause Applied Microbiology and Biotechnology Mass Spectrometry Excretion Feces chemistry.chemical_compound medicine Animals Petroleum ether Mycotoxin Chromatography Ecology Toxin Feces analysis Broiler Metabolism Chemistry T-2 Toxin chemistry Chromatography Thin Layer Chickens Sesquiterpenes Research Article Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 39:1172-1177 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.39.6.1172-1177.1980 |
| Popis: | A method for the detection of T-2 metabolites was developed and applied to analysis of metabolites in excreta of broiler chickens administered 3H-labeled T-2 toxin. The method used acetonitrile extraction and partitioning with petroleum ether followed by chromatography on Amberlite XAD-2, Florisil, and Sep-Pak C18. The recovery of T-2 toxin added to the chicken excreta was 73% at a concentration of 0.2 microgram/g. About 80% of orally administered 3H-labeled T-2 toxin was rapidly metabolized to more polar derivatives and eliminated in the excreta within 48 h. T-2 toxin, HT-2 toxin, neosolaniol, and T-2 tetraol were detected at 0.06 to 1.13% of the total dose, 48 h after administration. Eight unknown derivatives, named TB-1 to TB-8, were quantitatively more significant than the metabolites above. TB-3 and TB-9 represented about 12 and 25% of the total dose, respectively. One of the metabolites (TB-6), 1.5% of the total dose, was identified as 4-deacetylneosolaniol (15-acetyl-3 alpha, 4 beta, 8 alpha-trihydroxy-12, 13-epoxytrichothec-9-ene). |
| Databáze: | OpenAIRE |
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