Ultrasound-Guided Approaches to Improve Orthotopic Mouse Xenograft Models for Hepatocellular Carcinoma

Autor: Yann Herault, Emilie Thiebault, Thomas F. Baumert, Ghina Bou About, Tania Sorg, Laurent Monassier, Hugues Jacobs, Eric Robinet, Marie Wattenhofer-Donzé, Alain Guimond
Přispěvatelé: Institut de Recherche sur les Maladies Virales et Hépatiques (IVH), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire de neurobiologie et pharmacologie cardiovasculaire (LNPCV), Université de Strasbourg (UNISTRA), Laboratoire de pharmacologie et toxicologie neurocardiovasculaire (LPTNC), Institut de génétique et biologie moléculaire et cellulaire (IGBMC), Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA), Institut Clinique de la Souris (ICS), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), French National Infrastructure for Mouse Phenogenomics (PHENOMIN), Institut de Chirurgie guidée par l'Image, univOAK, Archive ouverte
Rok vydání: 2019
Předmět:
Cirrhosis
Huh-7
Sciences du Vivant [q-bio]/Cancer
Heterologous/instrumentation/*methods
Mice
0302 clinical medicine
Liver Neoplasms
Experimental
Ultrasonics
Stage (cooking)
0303 health sciences
Sciences du Vivant [q-bio]/Ingénierie biomédicale
Liver Neoplasms
Liver Neoplasms/therapy
Sciences du Vivant [q-bio]/Biotechnologies
General Medicine
3. Good health
Drug development
Hepatocellular carcinoma
Heterografts
medicine.drug
Carcinoma
Hepatocellular
Experimental/*therapy
Transplantation
Heterologous
[SDV.CAN]Life Sciences [q-bio]/Cancer
Ultrasonics/instrumentation/*methods
Hepatocellular/therapy
doxorubicin
03 medical and health sciences
[SDV.CAN] Life Sciences [q-bio]/Cancer
echo-guided injection
medicine
Carcinoma
Distribution (pharmacology)
Animals
Doxorubicin
orthotopic
030304 developmental biology
Heterografts/*physiology
Transplantation
business.industry
echography
medicine.disease
digestive system diseases
hepatocarcinoma
Cancer research
business
030217 neurology & neurosurgery
[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Zdroj: Current Protocols in Mouse Biology
Current Protocols in Mouse Biology, Wiley Online Library, 2019, 9 (2), ⟨10.1002/cpmo.62⟩
Current Protocols in Mouse Biology, 2019, 9 (2), ⟨10.1002/cpmo.62⟩
ISSN: 2161-2617
Popis: Hepatocellular carcinoma (HCC) is the second leading cause of cancer death worldwide. While curative approaches for early stage HCC exist, effective treatment options for advanced HCC are lacking. Furthermore, there are no efficient chemopreventive strategies to limit HCC development once cirrhosis is established. One challenge for drug development is unsatisfactory animal models. In this article, we describe an orthotopic xenograft mouse model of human liver cancer cell lines through image-guided injection into the liver. This technique provides a less invasive yet highly efficient approach to engraft human HCC into mouse liver. Similarly, image-guided injections are used to deliver chemotherapeutics locally, enabling reduction in potential systemic adverse effects, while reducing the required dose for a therapeutic effect. In summary, this image-guided strategy provides a novel and convenient approach to improve current HCC mouse models. (c) 2019 The Authors. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
Databáze: OpenAIRE
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