Human uterine lymphocytes acquire a more experienced and tolerogenic phenotype during pregnancy
Autor: | Marilen Benner, Renate G. van der Molen, Irma Joosten, Dorien Feyaerts, Bram van Cranenbroek, Olivier W.H. van der Heijden |
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Jazyk: | angličtina |
Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Science Decidua Parietalis lnfectious Diseases and Global Health Radboud Institute for Molecular Life Sciences [Radboudumc 4] Endometrium Peripheral blood mononuclear cell Article Immunophenotyping 03 medical and health sciences Immune system Pregnancy T-Lymphocyte Subsets medicine Decidua Immune Tolerance Humans Lymphocytes Menstrual blood Fetus Multidisciplinary business.industry Uterus medicine.disease Phenotype 030104 developmental biology medicine.anatomical_structure Cellular Microenvironment Immunology Medicine Female business Inflammatory diseases Radboud Institute for Molecular Life Sciences [Radboudumc 5] |
Zdroj: | Scientific Reports Scientific Reports, Vol 7, Iss 1, Pp 1-10 (2017) Scientific Reports, 7 |
ISSN: | 2045-2322 |
Popis: | Pregnancy requires a delicate immune balance that nurtures the allogeneic fetus, while maintaining reactivity against pathogens. Despite increasing knowledge, data is lacking on the transition of pre-pregnancy endometrial lymphocytes to a pregnancy state. Here, we immunophenotyped lymphocytes from endometrium (MMC), term decidua parietalis (DPMC), and PBMC for direct comparison. We found that the immune cell composition of MMC and DPMC clearly differ from each other, with less NK-cells, and more NKT-cells and T-cells in DPMC. An increased percentage of central memory and effector memory T-cells, and less naive T-cells in DPMC indicates that decidual T-cells are more experienced than endometrial T-cells. The increased percentage of CD4+CD25highCD127− Treg in DPMC, including differentiated Treg, is indicative of a more experienced and tolerogenic environment during pregnancy. The Th cell composition of both MMC and DPMC was different from PBMC, with a preference for Th1 over Th2 in the uterine environment. Between MMC and DPMC, percentages of Th cell subsets did not differ significantly. Our results suggest that already before pregnancy a tightly controlled Th1/Th2/Th17 balance is present. These findings create opportunities to further investigate the underlying immune mechanism of pregnancy complications using menstrual blood as a source for endometrial lymphocytes. |
Databáze: | OpenAIRE |
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