Visualizing Borrelia burgdorferi Infection Using a Small-Molecule Imaging Probe
| Autor: | Monica E. Embers, Nicole R. Hasenkampf, Neil L. Spector, Andrew E. Padilla, Havard S. Lambert, David A. Alcorta, Dakota W. Nollner, Madeline G Sell |
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| Rok vydání: | 2021 |
| Předmět: |
Diagnostic Imaging
0301 basic medicine Microbiology (medical) heat shock protein small molecule imaging infection Microbiology Mice 03 medical and health sciences 0302 clinical medicine Lyme disease Bacterial Proteins In vivo Heat shock protein medicine Animals Humans Borrelia burgdorferi Pathogen Lyme Disease biology Borrelia Burgdorferi Infection Bacteriology small-molecule probe medicine.disease biology.organism_classification 030104 developmental biology 030220 oncology & carcinogenesis biology.protein Protein G Bacteria |
| Zdroj: | Journal of Clinical Microbiology |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.02313-20 |
| Popis: | In vivo diagnostic imaging of bacterial infections is currently reliant on targeting their metabolic pathways, an ineffective method to identify microbial species with low metabolic activity. Here, we establish HS-198 as a small-molecule fluorescent conjugate that selectively targets the highly conserved bacterial protein HtpG (high-temperature protein G), within Borrelia burgdorferi, the bacterium responsible for Lyme disease. We describe the use of HS-198 to target morphologic forms of B. burgdorferi in both the logarithmic growth phase and the metabolically dormant stationary phase as well as in inactivated spirochetes. Furthermore, in a murine infection model, systemically injected HS-198 identified B. burgdorferi as revealed by imaging in postnecropsy tissue sections. These findings demonstrate how small-molecule probes directed at conserved bacterial protein targets can function to identify the microbe using noninvasive imaging and potentially as scaffolds to deliver antimicrobial agents to the pathogen. |
| Databáze: | OpenAIRE |
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