Measurement of intestinal permeability using lactulose and mannitol with conventional five hours and shortened two hours urine collection by two different methods: HPAE-PAD and LC-MSMS

Autor: Tahmeed Ahmed, Abdullah Siddique, Mamun Kabir, Dinesh Mondal, Mustafa Mahfuz, Rashidul Haque, Humaira Rashid, William A. Petri, Md. Abu Musa, Emtiaz Ahmed, Md. Iqbal Hossain
Rok vydání: 2019
Předmět:
Male
0301 basic medicine
Time Factors
Physiology
Urine
Gastroenterology
Lactulose
0302 clinical medicine
Medicine and Health Sciences
Ingestion
Mannitol
Intestinal Mucosa
Urine Specimen Collection
Multidisciplinary
Organic Compounds
Body Fluids
Chemistry
Child
Preschool
Physical Sciences
Medicine
Female
030211 gastroenterology & hepatology
Anatomy
Research Article
medicine.drug
Urine collection
medicine.medical_specialty
Colon
Science
Materials Science
Material Properties
Excretion
Gastroenterology and Hepatology
Permeability
03 medical and health sciences
Internal medicine
medicine
Humans
Intestinal permeability
business.industry
Organic Chemistry
Inflammatory Bowel Disease
Chemical Compounds
Infant
Biology and Life Sciences
medicine.disease
Gastrointestinal Tract
Intestinal Diseases
030104 developmental biology
Intestinal Absorption
Alcohols
Physiological Processes
business
Digestive System
Zdroj: PLoS ONE
PLoS ONE, Vol 14, Iss 8, p e0220397 (2019)
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0220397
Popis: Urinary excretion of two orally-administered non-metabolizable sugars, lactulose and mannitol, is a valuable marker for evaluating intestinal permeability. Usually this test involves a time consuming procedure of about 5 hour's urine collection, which makes the test incompatible to some extent. As the results are expressed as the ratio of lactulose and mannitol recovered in urine within certain time, it may be possible to get similar result despite the reduced urine collection time of 2 hours. Moreover, different laboratories do the test by different methods, which make the results incomparable between laboratories. Here, we are also trying to find the correlation between results from most commonly used methods: HPAE-PAD and LC-MSMS. The lactulose: mannitol (LM) test was performed in a cohort of Bangladeshi infants considered at-risk for environmental enteropathy. 208 urine specimens from 104 (52 male and 52 female) infants were collected at 2 and 5 hours after LM solution administration and were tested for lactulose and mannitol by two different methods, one HPAE-PAD platform and another LC-MSMS platform. Median age of the children was 15.0 months (range 6.9 to 25.8 months) and their mean weight-for-age z-score was -0.92. A higher percentage of lactulose and mannitol recovery was found in 5 hours urine collection than in the corresponding 2 hours by both HPAE-PAD and LC-MSMS method, but when results were expressed as lactulose to mannitol ratio (LMR) there was no significant difference between 2 and 5 hours urine collection in both HPAE-PAD (P = 0.138) and LC-MSMS (P = 0.099) method. LMR based on 2 hours urine collection correlated well with LMR based on traditional 5 hours urine collection (Spearman's correlation coefficient 0.578 and 0.604 respectively for HPAE-PAD and LC-MSMS). In future, LM test to assess intestinal permeability in children can be simplified by shortening the urine collection time from 5 hours to 2 hours.
Databáze: OpenAIRE
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