Cell wall modifications by α-XYLOSIDASE1 are required for control of seed and fruit size in Arabidopsis
| Autor: | Roberta Corti, Stefan de Folter, Lucia Colombo, Valeria Cassina, Nicola Babolin, Veronica Gregis, Edward Kiegle, Vívian Ebeling Viana, Francesco Mantegazza, Maurizio Di Marzo, Ignacio Ezquer, Javier Sampedro, Camilla Banfi, Andrea Guazzotti, Humberto Herrera-Ubaldo |
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| Přispěvatelé: | DI Marzo, M, Viana, V, Banfi, C, Cassina, V, Corti, R, Herrera-Ubaldo, H, Babolin, N, Guazzotti, A, Kiegle, E, Gregis, V, De Folter, S, Sampedro, J, Mantegazza, F, Colombo, L, Ezquer, I |
| Rok vydání: | 2021 |
| Předmět: |
Physiology
Arabidopsis Plant Science Polysaccharide Cell wall chemistry.chemical_compound xyloglucan Arabidopsis thaliana Hemicellulose seed size Transcription factor transcription factor chemistry.chemical_classification biology Arabidopsis Proteins Cell growth food and beverages biology.organism_classification Cell biology Cellulose microfibril Xyloglucan chemistry Fruit Seeds MADS-box fruit growth |
| Zdroj: | Journal of Experimental Botany. 73:1499-1515 |
| ISSN: | 1460-2431 0022-0957 |
| Popis: | Cell wall modifications are of pivotal importance during plant development. Among cell wall components, xyloglucans are the major hemicellulose polysaccharide in primary cell walls of dicots and non-graminaceous monocots. They can connect the cellulose microfibril surface to affect cell wall mechanical properties. Changes in xyloglucan structure are known to play an important role in regulating cell growth. Therefore, the degradation of xyloglucan is an important modification that alters the cell wall. The α-XYLOSIDASE1 (XYL1) gene encodes the only α-xylosidase acting on xyloglucans in Arabidopsis thaliana. Here, we showed that mutation of XYL1 strongly influences seed size, seed germination, and fruit elongation. We found that the expression of XYL1 is directly regulated in developing seeds and fruit by the MADS-box transcription factor SEEDSTICK. We demonstrated that XYL1 complements the stk smaller seed phenotype. Finally, by atomic force microscopy, we investigated the role of XYL1 activity in maintaining cell stiffness and growth, confirming the importance of cell wall modulation in shaping organs. |
| Databáze: | OpenAIRE |
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