Integration of clinical and gene expression endpoints to explore furan-mediated hepatotoxicity
| Autor: | Jeff W. Chou, Elias T. Gaillard, Raymond E. Stoll, Kerry T. Blanchard, Pierre R. Bushel, Hisham K. Hamadeh, Charles J. Tucker, Qihong Huang, Jennifer B. Collins, Robert R. Maronpot, Supriya Jayadev, Cynthia A. Afshari |
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| Rok vydání: | 2004 |
| Předmět: |
Male
DNA Complementary Microarray Health Toxicology and Mutagenesis Inflammation Biology Bioinformatics Rats Sprague-Dawley Fibrosis Gene expression Genetics medicine Animals Furans Molecular Biology Oligonucleotide Array Sequence Analysis Gene Expression Profiling RNA medicine.disease Phenotype Rats Liver Toxicity Cancer research medicine.symptom Toxicogenomics |
| Zdroj: | Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 549:169-183 |
| ISSN: | 0027-5107 |
| Popis: | Molecular techniques, such as cDNA microarrays, are being used to aid in the elucidation of the mechanisms of toxicity of a variety of compounds. In this study, we evaluate the molecular effects of furan in the rat liver. Sprague-Dawley rats were exposed to 4 or 40 mg/kg furan for up to 14 days. Furan induced an initial degenerative and necrotic phenotype that was followed by inflammation and fibrosis, consistent with previous observations for this compound. RNA was harvested from each lobe of the liver at several time points to observe whether lobe-specific gene expression effects occurred. Similar gene expression changes were observed in all lobes, however the magnitude of gene expression change was more pronounced in the right lobe. Finally, to help determine the correlation between gene expression changes and liver pathology, we applied traditional microarray visualization tools to the assessment of clinical chemistry and pathology parameters. |
| Databáze: | OpenAIRE |
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