Antigens specified by herpesviruses
| Autor: | Richard J. Courtney, Matilda Benyesh-Melnick, Robert M. McCombs |
|---|---|
| Rok vydání: | 1971 |
| Předmět: |
Antiserum
Simplexvirus food.ingredient medicine.diagnostic_test Arginine Biology Immunofluorescence medicine.disease_cause Virology Molecular biology Virus Microbiology Cell nucleus food medicine.anatomical_structure Herpes simplex virus Viral replication Antigen Biochemistry Cell culture Cytoplasm medicine Nuclear protein Polyacrylamide gel electrophoresis |
| Zdroj: | Virology. 43:356-365 |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/0042-6822(71)90308-4 |
| Popis: | The synthesis of herpes simplex virus proteins in the presence of arginine or under conditions of arginine deprivation was followed by polyacrylamide gel electrophoresis. When cells grown in the presence of arginine were pulse-labeled 30 min prior to harvesting, the synthesis of at least 11 distinct cytoplasmic proteins could be followed at various stages (2–10 hours postinfection) of the infective cycle. Arginine-deprived cultures synthesized the same cytoplasmic proteins, but in smaller amounts. A difference was noted in the time of appearance of cytoplasmic proteins C4 and C5. In the presence of arginine protein C5 was more predominant during the early stages of infection, whereas C4 was the major protein synthesized during the late stages of the infectious cycle. In contrast, in cultures devoid of arginine, protein C5 was the predominant one of these two proteins during both the early and late stages of infection. Direct precipitation experiments with herpes antisera indicated that proteins C4 and C5 were virus specific. The events which occur within the nuclear fraction of infected cells after addition of arginine to the arginine-deprived cultures were followed by double label pulse-chase experiments. Labeled herpes viral proteins, synthesized prior to the addition of arginine, were found to appear within the nuclear fraction only after arginine was added. Of the 3 major nuclear proteins (N1, N2, and N3), protein N2 appeared to be most affected by arginine deprivation. Cytoplasmic proteins C3, C4, and C5 were found to coelectrophorese with nuclear proteins N1, N2, and N3, respectively. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|