Tracking the amphibian pathogens Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans using a highly specific monoclonal antibody and lateral-flow technology

Autor: Jamie R. Stevens, Michael J Bungard, Michelle F. O'Brien, Christopher R. Thornton, Andrew E. Bowkett, Michael J. Dillon, Kieran A. Bates, Matthew C. Fisher, Katie M. Beckman
Přispěvatelé: Natural Environment Research Council (NERC)
Rok vydání: 2016
Předmět:
0301 basic medicine
YEAST POPULATION-DYNAMICS
Batrachochytrium salamandrivorans
Applied Microbiology and Biotechnology
Biochemistry
Chromatography
Affinity
Pathogen
Research Articles
biology
Antibodies
Monoclonal
GRAPE BERRIES
SULFUR-DIOXIDE
medicine.drug_formulation_ingredient
Chytridiomycota
WINE FERMENTATION
Antibodies
Fungal/immunology
Diagnostic Tests
Routine/methods
Immunochromatography
Antibody
Life Sciences & Biomedicine
Immunoglobulin M/immunology
0605 Microbiology
Research Article
Biotechnology
Veterinary Medicine
Amphibian
Antigens
Fungal
030106 microbiology
Urodela
Bioengineering
NON-SACCHAROMYCES
Microbiology
03 medical and health sciences
Antigen
biology.animal
medicine
Animals
BOTRYTIS-CINEREA
Chytridiomycosis
FUNGICIDE TREATMENTS
Antibodies
Fungal
Antibodies
Monoclonal/immunology
Science & Technology
Mycoses/diagnosis
Diagnostic Tests
Routine
biology.organism_classification
Antigens
Fungal/analysis
030104 developmental biology
Biotechnology & Applied Microbiology
Immunoglobulin M
Mycoses
Urodela/microbiology
Veterinary Medicine/methods
KLOECKERA-APICULATA
biology.protein
ANTIOXIDANT ACTIVITY
Chytridiomycota/isolation & purification
MICROBIAL DIVERSITY
Chromatography
Affinity/methods
Zdroj: Dillon, M J, Bowkett, A E, Bungard, M J, Beckmann, K, O'Brien, M F, Bates, K, Fisher, M C, Stevens, J R & Thornton, C R 2017, ' Tracking the amphibian pathogens Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans using a highly specific monoclonal antibody and lateral-flow technology ', Microbial biotechnology, vol. 10, no. 2, pp. 381-394 . https://doi.org/10.1111/1751-7915.12464
Microbial Biotechnology
ISSN: 1751-7915
Popis: Summary The fungus Batrachochytrium dendrobatidis (Bd) causes chytridiomycosis, a lethal epizootic disease of amphibians. Rapid identification of the pathogen and biosecurity is essential to prevent its spread, but current laboratory‐based tests are time‐consuming and require specialist equipment. Here, we describe the generation of an IgM monoclonal antibody (mAb), 5C4, specific to Bd as well as the related salamander and newt pathogen Batrachochytrium salamandrivorans (Bsal). The mAb, which binds to a glycoprotein antigen present on the surface of zoospores, sporangia and zoosporangia, was used to develop a lateral‐flow assay (LFA) for rapid (15 min) detection of the pathogens. The LFA detects known lineages of Bd and also Bsal, as well as the closely related fungus Homolaphlyctis polyrhiza, but does not detect a wide range of related and unrelated fungi and oomycetes likely to be present in amphibian habitats. When combined with a simple swabbing procedure, the LFA was 100% accurate in detecting the water‐soluble 5C4 antigen present in skin, foot and pelvic samples from frogs, newts and salamanders naturally infected with Bd or Bsal. Our results demonstrate the potential of the portable LFA as a rapid qualitative assay for tracking these amphibian pathogens and as an adjunct test to nucleic acid‐based detection methods.
Databáze: OpenAIRE
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