Comparative muscle transcriptome associated with carcass traits of Nellore cattle
| Autor: | Júlio Cesar de Carvalho Balieiro, Luiz Lehmann Coutinho, Aline Silva Mello Cesar, Bárbara Silva-Vignato, Mirele Daiana Poleti, Luciana Correia de Almeida Regitano |
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| Přispěvatelé: | Bárbara Silva Vignato, FZEA/USP, Luiz Lehmann Coutinho, ESALQ/USP, Aline Silva Mello Cesar, ESALQ/USP, Mirele Daiana Poleti, ESALQ/USP, LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE, Júlio Cesar de Carvalho Balieiro, FZEA/USP. |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
lcsh:QH426-470 lcsh:Biotechnology Paraspinal Muscles CARNES E DERIVADOS Beef cattle Biology Breeding Bos taurus indicus Backfat thickness Transcriptome 03 medical and health sciences Macromolecule modification Lipid biosynthesis Lipid droplet lcsh:TP248.13-248.65 Genetics Animals RNA-Seq KEGG Animal Husbandry Ribeye area Sequence Analysis RNA 0402 animal and dairy science 04 agricultural and veterinary sciences 040201 dairy & animal science Metabolic pathway lcsh:Genetics 030104 developmental biology Phenotype Cellular protein modification process Cattle Mitogen-Activated Protein Kinases Metabolic Networks and Pathways Biotechnology Research Article Signal Transduction |
| Zdroj: | BMC Genomics, Vol 18, Iss 1, Pp 1-13 (2017) BMC Genomics Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice) Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
| ISSN: | 1471-2164 |
| DOI: | 10.1186/s12864-017-3897-x |
| Popis: | Background Commercial cuts yield is an important trait for beef production, which affects the final value of the products, but its direct determination is a challenging procedure to be implemented in practice. The measurement of ribeye area (REA) and backfat thickness (BFT) can be used as indirect measures of meat yield. REA and BFT are important traits studied in beef cattle due to their strong implication in technological (carcass yield) and nutritional characteristics of meat products, like the degree of muscularity and total body fat. Thus, the aim of this work was to study the Longissimus dorsi muscle transcriptome of Nellore cattle, associated with REA and BFT, to find differentially expressed (DE) genes, metabolic pathways, and biological processes that may regulate these traits. Results By comparing the gene expression level between groups with extreme genomic estimated breeding values (GEBV), 101 DE genes for REA and 18 for BFT (false discovery rate, FDR 10%) were identified. Functional enrichment analysis for REA identified two KEGG pathways, MAPK (Mitogen-Activated Protein Kinase) signaling pathway and endocytosis pathway, and three biological processes, response to endoplasmic reticulum stress, cellular protein modification process, and macromolecule modification. The MAPK pathway is responsible for fundamental cellular processes, such as growth, differentiation, and hypertrophy. For BFT, 18 biological processes were found to be altered and grouped into 8 clusters of semantically similar terms. The DE genes identified in the biological processes for BFT were ACHE, SRD5A1, RSAD2 and RSPO3. RSAD2 has been previously shown to be associated with lipid droplet content and lipid biosynthesis. Conclusion In this study, we identified genes, metabolic pathways, and biological processes, involved in differentiation, proliferation, protein turnover, hypertrophy, as well as adipogenesis and lipid biosynthesis related to REA and BFT. These results enlighten some of the molecular processes involved in muscle and fat deposition, which are economically important carcass traits for beef production. Electronic supplementary material The online version of this article (doi:10.1186/s12864-017-3897-x) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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