| Popis: |
Pediococcus pentosaceus is a kind of beneficial lactic acid bacteria (LAB), which can inhibit foodborne pathogens, regulate the intestinal immune function, reduce cholesterol level, anti-tumor, inhibit microorganisms and other probiotic functions. In this study, Pseudomonas aeruginosa was used as the indicator bacteria to investigate the mechanism of the antagonistic effect of P. pentosaceus (Rsp5 and Rsp6) against P. aeruginosa by methods of test the antibacterial ability, pyocyanin expression, biofilm formation, hydrophobicity and auto-aggregation of P. pentosaceus and co-aggregation ability between the two strains. The results showed that two strains of P. pentosaceus (Rsp5 and Rsp6) had a significant antagonistic ability against P. aeruginosa, but the antagonistic mechanism was different. Rsp5 can inhibit the growth of P. aeruginosa (inhibition zone is 2.013±0.194 cm), inhibit biofilm formation (inhibition rate is about 55%), and reduce the infectivity of P. aeruginosa through its own co-agglutination with P. aeruginosa PAO1. Rsp6 can inhibit the growth of P. aeruginosa (inhibition zone is 1.907±0.272 cm), the expression of pyocyanin (inhibition rate is about 47%) and biofilm formation (inhibition rate is about 60%). This study will provide a research basis to prevent P. aeruginosa by LAB. |
