Transport of 3-O-methylglucose in isolated rat retinal pigment epithelial cells
| Autor: | Lawrence E. Stramm, E.L. Pautler |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Phloretin
Stereochemistry 3-O-Methylglucose In Vitro Techniques Ouabain Cellular and Molecular Neuroscience chemistry.chemical_compound Pigment medicine Animals Pigment Epithelium of Eye Methylglycosides Retinal pigment epithelium Glucose transporter Methylglucosides Biological Transport Retinal Molecular biology Sensory Systems Rats Dinitrobenzenes Kinetics Ophthalmology Glucose medicine.anatomical_structure chemistry visual_art Dinitrophenol visual_art.visual_art_medium medicine.drug |
| Zdroj: | Experimental Eye Research. 35:91-97 |
| ISSN: | 0014-4835 |
| DOI: | 10.1016/s0014-4835(82)80057-2 |
| Popis: | The uptake of 3-O-methylglucose in isolated rat retinal pigment epithelial cells exhibits complex kinetics as revealed by a plot of the Lineweaver-Burke transformation of the Michaelis-Menten equation. Conventional analysis of the linear portion of the double-reciprocal plot yields a Km of 7 . 69 mM and a Vmax of 1 . 95 nM/mg protein/min. If data are analyzed to take into account the existence of two carrier-mediated processes, a high affinity system (Km = 1 . 94 mM, Vmax = 0 . 012 nM/mg protein/min) and a low affinity system (Km = 640 mM, Vmax = 12 . 20 nM/mg protein/min) for 3-O-methylglucose uptake results. Three-O-methylglucose accumulation over a 10-min period was insensitive to phloridzin (0 . 1 mM), ouabain (1 mM) and dinitrophenol (1 mM), but was inhibited by mercuric chloride (1 mM). Phloretin (0 . 1 mM) increased the accumulation of both glucose and 3-O-methylglucose, which may indicate the existence of carrier-mediated efflux system. These findings demonstrate that glucose transport in rat retinal pigment epithelial cells is a complex process involving carrier mediation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|