Global and Regional Damages in Retinal Ganglion Cell Axon Bundles Monitored Non-Invasively by Visible-Light Optical Coherence Tomography Fibergraphy
| Autor: | Marta Grannonico, Mingna Liu, Hao Zhang, Pedro Norat, Peter A. Netland, Xiaorong Liu, Christopher D. Deppmann, David Miller |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
Retinal Ganglion Cells genetic structures Nerve fiber Neuroimaging Biology Retinal ganglion chemistry.chemical_compound Mice Optical coherence tomography medicine Animals Axon Research Articles Retina medicine.diagnostic_test General Neuroscience Retinal eye diseases Axons Mice Inbred C57BL medicine.anatomical_structure Retinal ganglion cell chemistry Optic nerve Female sense organs Neuroscience Tomography Optical Coherence |
| Zdroj: | J Neurosci |
| Popis: | Retinal ganglion cells (RGCs) exhibit compartmentalized organization, receiving synaptic inputs through their dendrites and transmitting visual information from the retina to the brain through the optic nerve. Little is known about the structure of RGC axon bundles extending from individual RGC somas to the optic nerve head (ONH) and how they respond to disease insults. We recently introduced visible-light optical coherence tomography fibergraphy (vis-OCTF), a technique for directly visualizing and analyzing mouse RGC axon bundlesin vivo. In this study, we validated vis-OCTF's ability to quantify RGC axon bundles with an increased number of RGCs using mice deficient in BCL2-associated X protein (BAX−/−). Next, we performed optic nerve crush (ONC) injury on wild-type (WT) mice and showed that the changes in RGC axon bundle width and thickness were location-dependent. Our work demonstrates the potential of vis-OCTF to longitudinally quantify and track RGC damage at single axon bundle level in optic neuropathies.SIGNIFICANCE STATEMENTNearly all clinical and preclinical studies measure the retinal nerve fiber (RNFL) thickness as the sole indicator of retinal ganglion cell (RGC) damage without investigating RGC axon bundles directly. We demonstrated visible-light optical coherence tomography fibergraphy (vis-OCTF) to directly quantify global and regional RGC axon bundle organizationsin vivoas a new biomarker for RGC health. We validatedin vivovis-OCTF measures using both confocal microscopy of the immunostained flat-mounted retina and numerical simulations. Vis-OCTF for monitoring RGC axon bundle organization has the potential to bring new insight into RGC damage in optic neuropathies. |
| Databáze: | OpenAIRE |
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