Bacteriological Assessment of the Indoor Air of Different Hospitals of Kathmandu District
| Autor: | Shyaron Poudel, Pramila Parajuli, Samyukta Tamrakar, Arzu Kunwar, Sony Sharma |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Veterinary medicine Cetrimide agar Article Subject 030106 microbiology 010501 environmental sciences 01 natural sciences Microbiology law.invention Agar plate 03 medical and health sciences chemistry.chemical_compound law Medicine media_common.cataloged_instance Mannitol salt agar European union 0105 earth and related environmental sciences media_common business.industry QR1-502 Gram staining chemistry Gentamicin business MacConkey agar Nutrient agar medicine.drug Research Article |
| Zdroj: | International Journal of Microbiology, Vol 2019 (2019) International Journal of Microbiology |
| ISSN: | 1687-9198 |
| Popis: | Nosocomial infection is the infection that has been caught in a hospital and is potentially caused by organisms that are not susceptible to antibiotics. Nosocomial infections are transmitted directly or indirectly through air and may cause different types of infections. This study was undertaken with an objective to determine the prevalence of nosocomial bacteria present in hospital indoor environment. A total of 16 air samples were taken from general wards and emergency wards of 8 different hospitals using an impactor air sampler in nutrient agar, mannitol salt agar, blood agar, cetrimide agar, and MacConkey agar. The bacteriological agents were isolated and identified by cultural characteristics, Gram staining, and biochemical tests, and their antibiotic susceptibility pattern was determined using CLSI Guideline, 2015. According to the European Union Guidelines to Good Manufacturing Practices, the hospitals were under C- and D-grade air quality. According to the European Commission, most of the hospitals were intermediately polluted. Out of 16 indoor air samples, 47.18% ofStaphylococcus aureusand 1.82%Pseudomonasspp. were isolated. CoNS,Streptococcusspp.,Micrococcusspp., andBacillusspp. and Gram-negative bacteriaE.coliandProteusspp. were identified. The bacterial load was found to be high in the emergency ward (55.8%) in comparison to that in the general ward (44.2%). There is statistically no significant difference between bacterial load and 2 wards (general and emergency) of different hospitals and among different hospitals. The most effective antibiotic against S. aureuswas gentamicin (81.81%) and ofloxacin (81.81%). Among the antibiotics used forPseudomonasspp., ceftriaxone (83.3%) and ofloxacin (83.3%) were effective. High prevalence ofS. aureusand Gram-negative bacteria was found in this study; it is therefore important to monitor air quality regularly at different hospitals to prevent HAI. |
| Databáze: | OpenAIRE |
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