A new oxidative stress indicator: Effect of 5-hydroxytryptophan on thiol-disulfide homeostasis in exercise
| Autor: | Yildirim Kayacan, Günay Cerit, Hayrullah Yazar, Babak Elmi Ghojebeigloo |
|---|---|
| Přispěvatelé: | Ondokuz Mayıs Üniversitesi, Kayacan, Y, Yazar, H, Cerit, G, Ghojebeigloo, BE, Sakarya Üniversitesi/Tıp Fakültesi/Temel Tıp Bilimleri Bölümü, Yazar, Hayrullah |
| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Serotonin medicine.medical_specialty Endocrinology Diabetes and Metabolism 030209 endocrinology & metabolism medicine.disease_cause Thiol disulfide homeostasis 5-Hydroxytryptophan Disulfide 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Physical Conditioning Animal Internal medicine medicine Animals Homeostasis Rats Wistar Exercise chemistry.chemical_classification 030109 nutrition & dietetics Nutrition and Dietetics Nutrition & Dietetics Thiol oxidation Disulfide bond Protein Disulfide Reductase (Glutathione) Repeatability Rats Oxidative Stress Endocrinology chemistry 5-hydroxytryptophan Oxidative stress Thiol |
| Zdroj: | Nutrition. :114-119 |
| ISSN: | 0899-9007 |
| Popis: | WOS: 000471735300018 PubMed: 30939385 Objectives: The aim of the present study was to evaluate the relationship between exercise and both 5-hydroxy-tryptophan and oxidative stress using thiol-disulfide homeostasis via what is likely a novel biomarker. Methods: Male albino Wistar rats (n = 32) were randomly divided into four groups as follows: control, exercise group, 5-hydroxytryptophan group (5H), and 5-HTP + exercise group (5Hex). Exercise and 5-HTP administration (25 mg/kg per d) were performed 5 d/wk for 10 wk. After completion of the experimental protocol, to determine oxidative stress parameters, serum total thiol and native thiol concentrations were measured. Dynamic disulfide status, reduced thiol, oxidized thiol (OT), and thiol oxidation reduction percentage ratios were compared between the groups. The methods used in the present study to measure dynamic thiol-disulfide homeostasis as calorimetric and duplex quantities were developed in 2014. These new methods are simple, reliable, and sensitive, with both high linearity and repeatability. Results: Compared with the control group, serum dynamic disulfide levels were significantly lower in the 5H group and highest in the control group. The lowest OT and the highest reduced thiol rates were determined to be in the 5H group. The highest OT value was found in the 5Hex group. Thiol oxidation reduction values were found to be highest in the 5H group and lowest in the 5Hex group. Conclusions: Both 5-HTP and moderate exercise seem to be significantly effective in inhibiting oxidative damage. In addition, the new oxidative stress measurement method used in this study is a promising practical and useful method to evaluate and improve the performance of athletes. (C) 2019 Elsevier Inc. All rights reserved. |
| Databáze: | OpenAIRE |
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