Significant hepatic expression of IL-2 and IL-8 in biliary atresia compared with other neonatal cholestatic disorders
| Autor: | Omima M. Abdel Haie, Mostafa Mohamed Sira, Reda Sanad Arafa, Dina S El-Azab, Amira Mohamed Noureldin Abdelrahman |
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| Rok vydání: | 2016 |
| Předmět: |
Male
Pathology medicine.medical_specialty Immunology Inflammation Biochemistry Gastroenterology Proinflammatory cytokine Pathogenesis 03 medical and health sciences 0302 clinical medicine Cholestasis Biliary Atresia Biliary atresia Internal medicine medicine Humans Immunology and Allergy Neonatal cholestasis Molecular Biology Retrospective Studies business.industry Liver Diseases Interleukin-8 Infant Newborn Infant Interleukin Hematology medicine.disease Liver 030220 oncology & carcinogenesis Ferritins Interleukin-2 Female 030211 gastroenterology & hepatology medicine.symptom business Immunostaining |
| Zdroj: | Cytokine. 79:59-65 |
| ISSN: | 1043-4666 |
| Popis: | Although the exact etiology of biliary atresia (BA) is still elusive, inflammation plays a key role. Release of proinflammatory cytokines from activated immune cells perpetuates the injury and causes biliary destruction. We aimed to study interleukin (IL)-2 and IL-8 expression in liver tissue of BA patients compared with other neonatal cholestatic disorders.The study included 59 infants with neonatal cholestasis in two groups; BA group (n=31) and non-BA group (n=28) with cholestatic disorders other than BA as controls. Demographic, clinical, laboratory, and histopathological parameters were collected. IL-2 and IL-8 immunostaining was performed. Immunostaining in portal cellular infiltrate was scored as positive or negative and expressed as the mean cell count in three portal tracts.The mean value of IL-2 and IL-8 positive inflammatory cells was significantly higher in BA than in non-BA group (P-values of 0.004 and 0.002 respectively). IL-2 correlated significantly with IL-8 immunostaining in both BA and non-BA group (P0.0001 for both). Furthermore, both cytokines in both groups correlated significantly with inflammatory activity in liver biopsy while there was no significant correlation with the other studied parameters. Yet, there was a trend of increased expression of IL-2 and IL-8 with increasing stage of fibrosis in BA group. This trend was not observed in non-BA group.The significantly higher expression of IL-2 and IL-8 in patients with BA compared to non-BA suggests a potential role for these cytokines in the pathogenesis in therapy of this devastating neonatal hepatic disorder. |
| Databáze: | OpenAIRE |
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