Protamine-mediated DNA coating remarkably improves bombardment transformation efficiency in plant cells
| Autor: | Rongda Qu, Robert K. Delong, Elumalai Sivamani |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
biology
food and beverages Oryza DNA Plant Science General Medicine Plants Plant cell Protamine Molecular biology Spermidine chemistry.chemical_compound Transformation (genetics) Transformation Genetic chemistry Gene expression Calcium chloride transformation biology.protein Biophysics Protamines Agronomy and Crop Science Plasmids Transformation efficiency |
| Zdroj: | Plant Cell Reports. 28:213-221 |
| ISSN: | 1432-203X 0721-7714 |
| DOI: | 10.1007/s00299-008-0636-4 |
| Popis: | We have developed a method by which remarkably higher efficiencies of transient and stable transformation were achieved in bombardment transformation of plants. Over fivefold increase in transient gus gene expression was achieved when rice or maize suspension cells were bombarded with gold particles coated with plasmid DNA in the presence of protamine instead of the conventional spermidine. A 3.3-fold improvement in stable transformation efficiency was also observed using rice suspension cells with the new coating approach. The coated protamine-plasmid DNA complex resisted degradation by a DNase or by rice cell extract much longer than the spermidine-plasmid DNA complex. The results from this study suggest that protamine protects plasmid DNA longer than spermidine when being delivered inside the cells, probably by forming a nano-scale complex, and thus helps improve the efficiency of particle bombardment-mediated plant transformation. |
| Databáze: | OpenAIRE |
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