Egr1 mediated the neuronal differentiation induced by extremely low-frequency electromagnetic fields
| Autor: | Jihye Moon, Yeju Seong, Jongpil Kim |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
animal structures
Cell- and Tissue-Based Therapy EGR1 Fluorescent Antibody Technique Disease Biology Immunofluorescence General Biochemistry Genetics and Molecular Biology Transcriptome Mice Electromagnetic Fields Gene expression medicine Animals Humans General Pharmacology Toxicology and Pharmaceutics Transcription factor Cells Cultured Early Growth Response Protein 1 Neurons medicine.diagnostic_test Reverse Transcriptase Polymerase Chain Reaction Cell Differentiation Mesenchymal Stem Cells Neurodegenerative Diseases General Medicine respiratory system Cell biology Transplantation Disease Models Animal Stem cell |
| Zdroj: | Life Sciences. 102:16-27 |
| ISSN: | 0024-3205 |
| DOI: | 10.1016/j.lfs.2014.02.022 |
| Popis: | Aim There is a specific frequency of extremely low-frequency electromagnetic field (ELF-EMF) that promotes neuronal differentiation. Although several mechanisms are known to regulate ELF-EMF-induced neuronal differentiation, a key factor that mediates neurogenic potentials by the ELF-EMF is largely unknown. Also, the potential use of ELF-EMF exposure in cell transplantation assays is yet to be determined, including their possible use in ELF-EMF based therapy of neurological diseases. The aim of this study is to understand the underlying mechanisms that mediate ELF-EMF-induced neuronal differentiation and also to harness these mechanisms for cell transplantation assays. Main method Human bone marrow–mesenchymal stem cells (hBM–MSCs) were exposed to ELF-EMF (50 Hz frequency, 1 mT intensity) for 8 days. The hBM–MSC derived neurons were then analyzed by general molecular biology techniques including immunofluorescence and quantitative RT-PCR. To assess changes in gene expression induced by ELF-EMF exposure, we analyzed the transcriptome of neuronal cells after an 8-day ELF-EMF exposure (50 Hz, 1 mT) and compared the transcriptional profiles to control cells. Key finding We found that early growth response protein 1 (Egr1) is one of the key transcription factors in ELF-EMF-induced neuronal differentiation. In addition, we show that transplantations of ELF-EMF-induced neurons significantly alleviate symptoms in mouse models of neurodegenerative disease. Significance These findings indicate that a specific transcriptional factor, Egr1, mediates ELF-EMF-induced neuronal differentiations, and demonstrate the promise of ELF-EMF based cell replacement therapies for neurodegenerative diseases. |
| Databáze: | OpenAIRE |
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