The identification of QTLs associated with the in vitro response of rye (Secale cereale L.)
| Autor: | Aneta Hromada-Judycka, Monika Rakoczy-Trojanowska, Anna Gruszczyńska, Hanna Bolibok |
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| Rok vydání: | 2007 |
| Předmět: |
Secale
Somatic embryogenesis QTL Quantitative Trait Loci Secale cereale L Population Genetic mapping Molecular marker Biology Quantitative trait locus Biochemistry Chromosomes Plant Tissue Culture Techniques Rye Tissue culture Inbred strain Immature inflorescences education Molecular Biology Crosses Genetic Genetics education.field_of_study Immature embryos Chromosome Mapping food and beverages Cell Biology biology.organism_classification Horticulture Callus Seeds Research Article Explant culture |
| Zdroj: | Cellular & Molecular Biology Letters |
| ISSN: | 1689-1392 |
| DOI: | 10.2478/s11658-007-0023-0 |
| Popis: | This study was conducted in order to identify quantitative trait loci (QTLs) for the in vitro culture response of winter rye (Secale cereale L.) immature embryos and immature inflorescences. A genetic linkage map comprising 67 SSRs, 9 ISSRs, 13 SAMPLs, 7 RAPDs, 2 SCARs and one EST marker was created based on the analyses of 102 recombinant inbred lines from the cross between lines L318 (which has a good response in tissue cultures) and L9 (which is unable to regenerate plants from somatic tissues and anthers). The map spans 979.2 cM, and the average distance between markers is 9.9 cM. Two characteristics were evaluated: callus induction (CI) and somatic embryogenesis ability (SE). They were expressed as the percentage of immature embryos/inflorescences producing callus (designated ECI/ICI) and the percentage of explants producing somatic embryos (ESE/ISE). All the analysed traits showed continuous variation in the mapping population but a non-normal frequency distribution. We identified nine putative QTLs controlling the tissue culture response of rye, explaining up to 41.6% of the total phenotypic variation: two QTLs for ECI — eci-1, eci-2; 4 for ESE — ece-1, ese-2, ese-3, ese-4; 2 for ICI — ici-1, ici2; and 1 for ISE — ise-1. They were detected on chromosomes 1R, 4R, 5R, 6R and 7R. |
| Databáze: | OpenAIRE |
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