Genome-wide sequencing and quantification of circulating microRNAs for dogs with congestive heart failure secondary to myxomatous mitral valve degeneration
| Autor: | Amy Bohan, SeungWoo Jung |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty 040301 veterinary sciences Real-Time Polymerase Chain Reaction 0403 veterinary science 03 medical and health sciences Myxomatous mitral valve degeneration Dogs Downregulation and upregulation Mitral valve microRNA Animals Medicine Clinical significance Circulating MicroRNA Dog Diseases Heart Failure Mitral Valve Prolapse General Veterinary Sequence Analysis RNA business.industry 04 agricultural and veterinary sciences General Medicine medicine.disease 030104 developmental biology medicine.anatomical_structure Real-time polymerase chain reaction Heart failure Mitral Valve business Biomarkers |
| Zdroj: | American Journal of Veterinary Research. 79:163-169 |
| ISSN: | 0002-9645 |
| Popis: | OBJECTIVE To characterize expression profiles of circulating microRNAs via genome-wide sequencing for dogs with congestive heart failure (CHF) secondary to myxomatous mitral valve degeneration (MMVD). ANIMALS 9 healthy client-owned dogs and 8 age-matched client-owned dogs with CHF secondary to MMVD. PROCEDURES Blood samples were collected before administering cardiac medications for the management of CHF. Isolated microRNAs from plasma were classified into microRNA libraries and subjected to next-generation sequencing (NGS) for genome-wide sequencing analysis and quantification of circulating microRNAs. Quantitative reverse transcription PCR (qRT-PCR) assays were used to validate expression profiles of differentially expressed circulating microRNAs identified from NGS analysis of dogs with CHF. RESULTS 326 microRNAs were identified with NGS analysis. Hierarchical analysis revealed distinct expression patterns of circulating microRNAs between healthy dogs and dogs with CHF. Results of qRT-PCR assays confirmed upregulation of 4 microRNAs (miR-133, miR-1, miR-let-7e, and miR-125) and downregulation of 4 selected microRNAs (miR-30c, miR-128, miR-142, and miR-423). Results of qRT-PCR assays were highly correlated with NGS data and supported the specificity of circulating microRNA expression profiles in dogs with CHF secondary to MMVD. CONCLUSIONS AND CLINICAL RELEVANCE These results suggested that circulating microRNA expression patterns were unique and could serve as molecular biomarkers of CHF in dogs with MMVD. |
| Databáze: | OpenAIRE |
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