Expression of dorsal-ventral genes during early development of Rhynchosciara americana embryos
| Autor: | J. C. Carvalho, C. E. Vanario-Alonso, Eliana Abdelhay, R.V. Bruno, D.N. Rocha |
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| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Central Nervous System
Embryo Nonmammalian animal structures Dorsal-ventral genes Physiology Immunology Biophysics Embryonic Development Genes Insect Ectoderm In situ hybridization Biochemistry Lower dipteran Axis patterning medicine Animals RNA Messenger General Pharmacology Toxicology and Pharmaceutics lcsh:QH301-705.5 Gene In Situ Hybridization Body Patterning Genetics lcsh:R5-920 biology Decapentaplegic Diptera General Neuroscience fungi Gene Expression Regulation Developmental Embryo Cell Biology General Medicine biology.organism_classification Early embryogenesis Cell biology medicine.anatomical_structure lcsh:Biology (General) Female Rhynchosciara americana Drosophila melanogaster lcsh:Medicine (General) Blastoderm |
| Zdroj: | Brazilian Journal of Medical and Biological Research, Volume: 38, Issue: 1, Pages: 27-31, Published: JAN 2005 Brazilian Journal of Medical and Biological Research, Vol 38, Iss 1, Pp 27-31 (2005) |
| Popis: | The establishment of dorsal-ventral polarity in Drosophila is a complex process which involves the action of maternal and zygotically expressed genes. Interspecific differences in the expression pattern of some of these genes have been described in other species. Here we present the expression of dorsal-ventral genes during early embryogenesis in the lower dipteran Rhynchosciara americana. The expression of four genes, the ventralizing genes snail (sna) and twist (twi) and the dorsalizing genes decapentaplegic (dpp) and zerknullt (zen), was investigated by whole-mount in situ hybridization. Sense and antisense mRNA were transcribed in vitro using UTP-digoxigenin and hybridized at 55 degrees C with dechorionated fixed embryos. Staining was obtained with anti-digoxigenin alkaline phosphatase-conjugated antibody revealed with NBT-BCIP solution. The results showed that, in general, the spatial-temporal expression of R. americana dorsal-ventral genes is similar to that observed in Drosophila, where twi and sna are restricted to the ventral region, while dpp and zen are expressed in the dorsal side. The differences encountered were subtle and probably represent a particular aspect of dorsal-ventral axis determination in R. americana. In this lower dipteran sna is expressed slightly later than twi and dpp expression is expanded over the lateral ectoderm during cellular blastoderm stage. These data suggest that the establishment of dorsal-ventral polarity in R. americana embryos follows a program similar to that observed in Drosophila melanogaster. |
| Databáze: | OpenAIRE |
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