NCAM140 is translocated into the nucleus by an importin-β1-dependent mechanism
| Autor: | Ingo Gotthard, Hilke Wobst, Andy Zielinski, Fatema Es-Saddiki, Christine Laurini, Mirka Homrich, Simone Diestel, Edda Stein |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cell Adhesion Molecules Neuronal Recombinant Fusion Proteins Active Transport Cell Nucleus Protein Array Analysis Gene Expression Importin Biology 03 medical and health sciences Cytosol Cell Line Tumor Chlorocebus aethiops Gene expression medicine Animals Humans Glutathione Transferase Cell Nucleus Neurons Cell Biology beta Karyopherins Transmembrane protein Rats Cell biology Protein Transport 030104 developmental biology medicine.anatomical_structure nervous system Cytoplasm COS Cells Neural cell adhesion molecule Nuclear transport Neural development Nucleus Protein Binding |
| Zdroj: | Experimental Cell Research. 371:372-378 |
| ISSN: | 0014-4827 |
| DOI: | 10.1016/j.yexcr.2018.08.031 |
| Popis: | The neural cell adhesion molecule (NCAM) is important for neural development and for plasticity in adult brain. Previous studies demonstrated a calmodulin-dependent import of a transmembrane fragment of NCAM into the nucleus that regulates gene expression. In a protein macroarray we identified importin-β1 as a potential interaction partner of NCAM's cytoplasmic tail. The interaction was verified and an importin-β1-dependent import of NCAM into the nucleus could be demonstrated using quantitative immunofluorescence analysis. Generation of NCAM deletion mutants revealed that the last amino acids of the cytoplasmic region of NCAM are dispensable whereas other parts of NCAM's cytoplasmic tail take part in its nuclear translocation. With this study we propose an alternative nuclear route for NCAM via the classical importin-mediated import. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect