A novel Drosophila antisense scaRNA with a predicted guide function
| Autor: | Giuseppe Tortoriello, Maria Furia, Maria Carmela Accardo, Mimmo Turano, Alberto Angrisani, Filippo Scialò |
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| Přispěvatelé: | Tortoriello, G., Accardo, M. C., Scialo, F., Angrisani, A., Turano, M., Furia, M., Tortoriello, Giuseppe, Scialò, F., Turano, Mimmo, Furia, Maria |
| Rok vydání: | 2009 |
| Předmět: |
Embryo
Nonmammalian RNA Untranslated Cajal body Molecular Sequence Data snRNA U1b Biology snoRNA Cell Line Transcriptome Transcription (biology) GAS5 Genetics Animals Drosophila Proteins RNA Small Nucleolar RNA Antisense Small nucleolar RNA Membrane Protein Gene In Situ Hybridization Fluorescence Regulator gene Base Sequence Animal Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling scaRNA Chromosome Mapping Computational Biology Gene Expression Regulation Developmental Membrane Proteins General Medicine Blotting Northern Non-coding RNA ncRNA Cell biology Drosophila melanogaster Mutation Drosophila Protein Drosophila Small nuclear RNA |
| Zdroj: | Gene. 436:56-65 |
| ISSN: | 0378-1119 |
| DOI: | 10.1016/j.gene.2009.02.005 |
| Popis: | A significant portion of eukaryotic small ncRNA transcriptome is composed by small nucleolar RNAs. From archaeal to mammalian cells, these molecules act as guides in the site-specific pseudouridylation or methylation of target RNAs. We used a bioinformatics search program to detect Drosophila putative orthologues of U79, one out of ten snoRNAs produced by GAS5, a human ncRNA involved in apoptosis, susceptibility to cancer and autoimmune diseases. This search led to the definition of a list of U79-related fly snoRNAs whose genomic organization, evolution and expression strategy are discussed here. We report that an intriguing novel specimen, named Dm46E3, is transcribed as a longer, unspliced precursor from the reverse strand of eiger, a fly regulatory gene that plays a key role in cell differentiation, apoptosis and immune response. Expression of Dm46E3 was found significantly up-regulated in a mutant strain in which eiger transcription is greatly reduced, suggesting that these two sense-antisense genes may be mutually regulated. Relevant to its function, Dm46E3 concentrated specifically in the Cajal bodies, followed a dynamic spatial expression profile during embryogenesis and displayed a degenerate antisense element that enables it to target U1b, a developmentally regulated isoform of the U1 spliceosomal snRNA that is particularly abundant in embryos. © 2008 Elsevier B.V. All rights reserved. |
| Databáze: | OpenAIRE |
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