JE Nakayama/JE SA14-14-2 virus structural region intertypic viruses: Biological properties in the mouse model of neuroinvasive disease
| Autor: | Janice Nickells, Thomas J. Chambers, Deborah A. Droll, William S. M. Wold, Xiaoshan Jiang |
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| Rok vydání: | 2007 |
| Předmět: |
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Neurovirulence viruses Neuroinvasiveness Virulence Genome Viral Biology Article Virus Mice 03 medical and health sciences Virology medicine Animals Cloning Molecular Encephalitis Japanese 030304 developmental biology Encephalitis Virus Japanese Neurons 0303 health sciences Base Sequence 030306 microbiology Flavivirus Viral encephalitis Japanese encephalitis medicine.disease biology.organism_classification Molecular biology Disease Models Animal Capsid DNA Viral Nervous System Diseases Vaccine Encephalitis |
| Zdroj: | Virology. 366:51-61 |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/j.virol.2007.04.016 |
| Popis: | A molecular clone of Japanese encephalitis (JE) virus Nakayama strain was used to create intertypic viruses containing either the 5′-C-prM-E or the prM-E region of the attenuated JE SA14-14-2 virus in the JE Nakayama background. These two intertypic JE viruses, JE-X/5′CprME(S) and JE-X/prME(S), respectively, generally resembled the parental JE virus in cell culture properties. Similar to virus derived from the JE Nakayama molecular clone (JE-XJN), JE-X/prME(S) was highly neuroinvasive and neurovirulent for young adult mice, whereas JE-X/5′CprME(S) was attenuated for neuroinvasiveness and only partially attenuated for neurovirulence. Immunization of young mice with JE-X/5′CprME(S) virus elicited neutralizing antibodies against JE Nakayama virus and conferred protection against encephalitis following challenge with JE Nakayama virus. The sequence of the JE-X/5′CprME(S) virus differed from that of JE-X/prME(S) virus at two nucleotides in the 5′ UTR, 3 amino acid positions in the capsid protein, 4 positions in the prM protein and 1 in the envelope protein. For JE-X/prME(S) virus, the 4 differences in prM and the single substitution in the envelope represented reversions to the sequence of JE Nakayama virus. Overall, this study reveals that molecular determinants associated with the prM-E region of the attenuated JE SA14-14-2 virus are insufficient by themselves to confer an attenuation phenotype upon JE Nakayama virus. This suggests a role for determinants in the 5′ UTR and/or the capsid protein of the JE SA 14-14-2 virus genome in influencing the virulence properties of the JE Nakayama virus in the mouse model. |
| Databáze: | OpenAIRE |
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