Streptococcus mutans Can Modulate Biofilm Formation and Attenuate the Virulence of Candida albicans
| Autor: | Simone Furgeri Godinho Vilela, Rodnei Dennis Rossoni, Marisol dos Santos Velloso, Júnia Oliveira Barbosa, Janaína Araújo de Alvarenga, Márcia Cristina de Azevedo Prata, Antonio Olavo Cardoso Jorge, Juliana Campos Junqueira |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Hyphal growth lcsh:Medicine Yeast and Fungal Models Pathogenesis Pathology and Laboratory Medicine Streptococcus mutans Larvae Candida albicans Medicine and Health Sciences lcsh:Science Candida Fungal Pathogens Multidisciplinary biology Candidiasis Corpus albicans Bacterial Pathogens Lepidoptera Infectious Diseases Medical Microbiology Larva Pathogens Anatomy Research Article Histology Hypha Urology 030106 microbiology Sexually Transmitted Diseases Virulence Mycology Research and Analysis Methods Microbiology 03 medical and health sciences Model Organisms In vivo Animals Humans Microbial Pathogens Bacteria Metamorphosis Genitourinary Infections lcsh:R fungi Biofilm Organisms Fungi Biology and Life Sciences Streptococcus Bacteriology biology.organism_classification Yeast Disease Models Animal 030104 developmental biology Biofilms Microbial Interactions lcsh:Q Bacterial Biofilms Developmental Biology |
| Zdroj: | PLoS ONE PLoS ONE, Vol 11, Iss 3, p e0150457 (2016) Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 1932-6203 |
| Popis: | Made available in DSpace on 2018-12-11T17:01:49Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-03-01 Streptococcus mutans and Candida albicans are found together in the oral biofilms on dental surfaces, but little is known about the ecological interactions between these species. Here, we studied the effects of S. mutans UA159 on the growth and pathogencity of C. albicans. Initially, the effects of S. mutans on the biofilm formation and morphogenesis of C. albicans were tested in vitro. Next, we investigate the influence of S. mutans on pathogenicity of C. albicans using in vivo host models, in which the experimental candidiasis was induced in G. mellonella larvae and analyzed by survival curves, C. albicans count in hemolymph, and quantification of hyphae in the host tissues. In all the tests, we evaluated the direct effects of S. mutans cells, as well as the indirect effects of the subproducts secreted by this microorganism using a bacterial culture filtrate. The in vitro analysis showed that S. mutans cells favored biofilm formation by C. albicans. However, a reduction in biofilm viable cells and inhibition of hyphal growth was observed when C. albicans was in contact with the S. mutans culture filtrate. In the in vivo study, injection of S. mutans cells or S. mutans culture filtrate into G. mellonella larvae infected with C. albicans increased the survival of these animals. Furthermore, a reduction in hyphal formation was observed in larval tissues when C. albicans was associated with S. mutans culture filtrate. These findings suggest that S. mutans can secrete subproducts capable to inhibit the biofilm formation, morphogenesis and pathogenicity of C. albicans, attenuating the experimental candidiasis in G. mellonella model. Department of Biosciences and Oral Diagnosis Univ Estadual Paulista/UNESP São José Dos Campos Embrapa Gado de Leite Juiz de for A Department of Biosciences and Oral Diagnosis Univ Estadual Paulista/UNESP São José Dos Campos |
| Databáze: | OpenAIRE |
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