Early Changes in Exo- and Endocytosis in the EAE Mouse Model of Multiple Sclerosis Correlate with Decreased Synaptic Ribbon Size and Reduced Ribbon-Associated Vesicle Pools in Rod Photoreceptor Synapses

Autor:	Karin Schwarz, Ekta Dembla, Mayur Dembla, Ajay Kesharwani, Frank Schmitz
Rok vydání:	2021
Předmět:	Dynamins rod photoreceptor Encephalomyelitis Autoimmune Experimental Multiple Sclerosis QH301-705.5 synaptic ribbon Central nervous system Endocytic cycle Ribbon synapse Endocytosis Catalysis Exocytosis Retina Article Inorganic Chemistry Synapse Mice Retinal Rod Photoreceptor Cells dynamin medicine Animals endocytosis Physical and Theoretical Chemistry Biology (General) Phosphorylation Molecular Biology QD1-999 Spectroscopy Dynamin phospho-dynamin Synaptic ribbon optic neuritis Chemistry Organic Chemistry General Medicine ribbon synapses Computer Science Applications Cell biology Mice Inbred C57BL Disease Models Animal medicine.anatomical_structure Synapses Female sense organs Synaptic Vesicles EAE mouse model
Zdroj:	International Journal of Molecular Sciences Volume 22 Issue 19 International Journal of Molecular Sciences, Vol 22, Iss 10789, p 10789 (2021)
ISSN:	1422-0067
Popis:	Multiple sclerosis (MS) is an inflammatory disease of the central nervous system that finally leads to demyelination. Demyelinating optic neuritis is a frequent symptom in MS. Recent studies also revealed synapse dysfunctions in MS patients and MS mouse models. We previously reported alterations of photoreceptor ribbon synapses in the experimental auto-immune encephalomyelitis (EAE) mouse model of MS. In the present study, we found that the previously observed decreased imunosignals of photoreceptor ribbons in early EAE resulted from a decrease in synaptic ribbon size, whereas the number/density of ribbons in photoreceptor synapses remained unchanged. Smaller photoreceptor ribbons are associated with fewer docked and ribbon-associated vesicles. At a functional level, depolarization-evoked exocytosis as monitored by optical recording was diminished even as early as on day 7 after EAE induction. Moreover compensatory, post-depolarization endocytosis was decreased. Decreased post-depolarization endocytosis in early EAE correlated with diminished synaptic enrichment of dynamin3. In contrast, basal endocytosis in photoreceptor synapses of resting non-depolarized retinal slices was increased in early EAE. Increased basal endocytosis correlated with increased de-phosphorylation of dynamin1. Thus, multiple endocytic pathways in photoreceptor synapse are differentially affected in early EAE and likely contribute to the observed synapse pathology in early EAE.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::58b3d43eb8bdf2a28caea1b26263e421 https://pubmed.ncbi.nlm.nih.gov/34639129 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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