A serum-free, chemically-defined medium for function and growth of primary neonatal rat heart cell cultures
| Autor: | S. N. W. Mohamed, R. Holmes, C. R. Hartzell |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
medicine.medical_specialty
Hydrocortisone Cell Survival medicine.medical_treatment Plant Science Biology Epidermal growth factor Internal medicine medicine Animals Bovine serum albumin Cells Cultured chemistry.chemical_classification Epidermal Growth Factor Myocardium Growth factor Rats Inbred Strains Myocardial Contraction Fetuin Culture Media Rats Thyroxine Chemically defined medium Endocrinology Animals Newborn chemistry Cell culture Transferrin biology.protein Cell Division Biotechnology Hormone |
| Zdroj: | In Vitro. 19:471-478 |
| ISSN: | 1475-2689 0073-5655 |
| DOI: | 10.1007/bf02619594 |
| Popis: | A serum-free, chemically defined medium for supporting rhythmic contraction, maximum survival, and moderate growth of cardiac cells was achieved by using a combination of hormones and growth supplements in a mixture of equal volumes of Ham's F12 and Dulbecco's modified Eagle's medium. The hormones and growth supplements included insulin, transferrin, selenium, fetuin, bovine serum albumin, hydrocortisone (HC), L-thyroxine (T4), and epidermal growth factor (EGF). Cardiac cells were grown on fibronectin-precoated plates using the above serum-free medium. Cells grown in this medium exhibited a higher beating rate and were maintained for a longer time compared to those cells grown in serum. The effects of T4, EGF, and HC on beating rate and survival time of both cultures of mixed cell population and enriched myoblast cell population were studied. In the enriched myoblast cell cultures grown in serum-supplemented medium, the beating rate ranged from 40 to 200 beats/min, and these cultures survived for 30 d. When these enriched cell cultures were grown in serum-free hormone-supplemented medium, the beating rate ranged from 190 to 240 beats/min, and these cultures survived for more than 90 d. These results show that some hormones affect growth, whereas others affect function. |
| Databáze: | OpenAIRE |
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