A cyclic GMP-dependent signalling pathway regulates bacterial phytopathogenesis
| Autor: | Shan-Ho Chou, Jane Rogers, Ko Hsin Chin, Yvonne McCarthy, Jauo Guey Yang, Melanie Febrer, David Swarbreck, J. Maxwell Dow, Chung Liang Liu, Shi-Qi An, Robert P. Ryan |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Cyclic di-GMP
0303 health sciences General Immunology and Microbiology 030306 microbiology Effector General Neuroscience Guanosine Plasma protein binding Biology Corrigenda General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound 03 medical and health sciences Protein structure 0302 clinical medicine chemistry Biochemistry Second messenger system biology.protein Diguanylate cyclase Signal transduction Molecular Biology 030217 neurology & neurosurgery 030304 developmental biology |
| Zdroj: | The EMBO Journal. 32:2779-2781 |
| ISSN: | 1460-2075 0261-4189 |
| DOI: | 10.1038/emboj.2013.215 |
| Popis: | Cyclic guanosine 3′,5′-monophosphate (cyclic GMP) is a second messenger whose role in bacterial signalling is poorly understood. A genetic screen in the plant pathogen Xanthomonas campestris (Xcc) identified that XC_0250, which encodes a protein with a class III nucleotidyl cyclase domain, is required for cyclic GMP synthesis. Purified XC_0250 was active in cyclic GMP synthesis in vitro. The linked gene XC_0249 encodes a protein with a cyclic mononucleotide-binding (cNMP) domain and a GGDEF diguanylate cyclase domain. The activity of XC_0249 in cyclic di-GMP synthesis was enhanced by addition of cyclic GMP. The isolated cNMP domain of XC_0249 bound cyclic GMP and a structure–function analysis, directed by determination of the crystal structure of the holo-complex, demonstrated the site of cyclic GMP binding that modulates cyclic di-GMP synthesis. Mutation of either XC_0250 or XC_0249 led to a reduced virulence to plants and reduced biofilm formation in vitro. These findings describe a regulatory pathway in which cyclic GMP regulates virulence and biofilm formation through interaction with a novel effector that directly links cyclic GMP and cyclic di-GMP signalling. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text