Mitotic and meiotic irregularities in somatic hybrids of Lycopersicon esculentum and Solanum tuberosum
| Autor: | Kamstra S, Maarten Koornneef, H.C.H. Schoenmakers, Eden J, Wolters Am, Jong Jh |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1994 |
| Předmět: |
Genetics
medicine.diagnostic_test biology fungi food and beverages Chromosome General Medicine biology.organism_classification Laboratorium voor Erfelijkheidsleer Lycopersicon Meiosis Chromosome Arm medicine Life Science Laboratory of Genetics Ploidy Molecular Biology Metaphase Biotechnology Fluorescence in situ hybridization Hybrid |
| Zdroj: | Scopus-Elsevier Genome 37 (1994) Genome, 37, 726-735 |
| ISSN: | 0831-2796 |
| DOI: | 10.1139/g94-103 |
| Popis: | Chromosome numbers were determined in metaphase complements of root-tip meristems of 107 tomato (+) potato somatic hybrids, obtained from five different combinations of parental genotypes. Of these hybrids 79% were aneuploid, lacking one or two chromosomes in most cases. All four hybrids that were studied at mitotic anaphase of root tips showed laggards and bridges, the three aneuploids in a higher frequency than the single euploid. Hybrid K2H2-1C, which showed the highest percentage of aberrant anaphases, possessed 46 chromosomes. Fluorescence in situ hybridization with total genomic DNA showed that this hybrid contained 23 tomato, 22 potato, and 1 recombinant chromosome consisting of a tomato chromosome arm and a potato chromosome arm. The potato parent of K2H2-1C was aneusomatic in its root tips with a high frequency of monosomic and trisomic cells and a relatively high frequency of cells with one fragment or telosome. Meiotic analyses of three tomato (+) potato somatic hybrids revealed laggards, which occurred most frequently in the triploid hybrids, and bridges, which were frequently present in pollen mother cells (PMCs) at anaphase I of hypotetraploid K2H2-1C. We observed putative trivalents in PMCs at diakinesis and metaphase I of eutriploid A7-82A and quadrivalents in part of the PMCs of hypotetraploid K2H2-1C, suggesting that homoeologous recombination between tomato and potato chromosomes occurred in these hybrids. All three hybrids showed a high percentage of first division restitution, giving rise to unreduced gametes. However, shortly after the tetrad stage all microspores completely degenerated, resulting in exclusively sterile pollen.Key words: tomato, potato, symmetric somatic hybrids, chromosomal irregularities, genomic in situ hybridization. |
| Databáze: | OpenAIRE |
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