Pseudopedobacter beijingensis gen. nov., sp. nov., isolated from coking wastewater activated sludge, and reclassification of Pedobacter saltans as Pseudopedobacter saltans comb. nov
| Autor: | Guizhen Li, Qiliang Lai, Zongze Shao, Junwei Cao |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
DNA
Bacterial China food.ingredient Molecular Sequence Data Pseudopedobacter Wastewater Biology medicine.disease_cause Microbiology Pseudopedobacter beijingensis food Genus RNA Ribosomal 16S Botany medicine Coke Phospholipids Phylogeny Ecology Evolution Behavior and Systematics Base Composition Sewage Strain (chemistry) Phylogenetic tree Bacteroidetes Phosphatidylethanolamines Fatty Acids Vitamin K 2 Sequence Analysis DNA General Medicine 16S ribosomal RNA biology.organism_classification Bacterial Typing Techniques Activated sludge Pedobacter Bacteria |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 64:1853-1858 |
| ISSN: | 1466-5034 1466-5026 |
| DOI: | 10.1099/ijs.0.053991-0 |
| Popis: | A taxonomic study was carried out on strain GCS-AE-31T, which was isolated from a phenol-degrading consortium, enriched from coking wastewater activated sludge of the Beijing Shougang Company Limited during the screening of phenol-degrading bacteria. Cells of strain GCS-AE-31T were Gram-stain-negative, short rods, motile by gliding, oxidase- and catalase-positive. Growth was observed at salinities of 0–3 % and at temperatures of 10–37 °C. On the basis of 16S rRNA gene sequence similarity, strain GCS-AE-31T was most closely related to Pedobacter saltans LMG 10337T (96.17 %), but it showed low similarity to all other species of the genus Pedobacter (89.28–92.45 %). It also showed low 16S rRNA gene similarity to all other species of the family Sphingobacteriaceae (87.25–92.45 %) examined. The dominant fatty acids were iso-C15 : 0, summed feature 3 (C16 : 1ω7c/C16 : 1ω6c), anteiso-C15 : 0 and iso-C17 : 0 3-OH. The menaquinones were MK-7 (95.5 %) and MK-6 (4.5 %). The polar lipids were phosphatidylethanolamine, three aminolipids and three unknown phospholipids. Sphingolipid was present. The G+C content of the chromosomal DNA was 36.2 mol%. According to its phylogenetic position and phenotypic traits, the novel strain could not be assigned to the genus Pedobacter ; it should be classified as representing a novel species of a novel genus in the family Sphingobacteriaceae , for which the name Pseudopedobacter beijingensis gen. nov., sp. nov. is proposed (type strain GCS-AE-31T = MCCC 1A01299T = CGMCC 1.12329T = LMG 27180T). The misclassified species Pedobacter saltans is transferred to the novel genus as Pseudopedobacter saltans comb. nov. (type strain LMG 10337T = MCCC 1A06472T = DSM 12145T = CCUG 39354T = CIP 105500T = JCM 21818T = NBRC 100064T). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|