Metabolomics profiling, biological activities, and molecular docking studies of elephant garlic (Allium ampeloprasum L.)
| Autor: | Ceren Emir, Gunes Coban, Ahmet Emir |
|---|---|
| Rok vydání: | 2022 |
| Předmět: |
Flavonoids
Crystal-Structure Antityrosinase activity Elephant garlic Anticholinesterase activity Antioxidant Activity Bioengineering Applied Microbiology and Biotechnology Biochemistry Tyrosinase Inhibitors In-Vitro Health Mushroom Tyrosinase Butyrylcholinesterase Molecular docking Acetylcholinesterase Phenolics Allium ampeloprasum Alzheimers-Disease |
| Zdroj: | Process Biochemistry. 116:49-59 |
| ISSN: | 1359-5113 |
| DOI: | 10.1016/j.procbio.2022.03.002 |
| Popis: | Allium ampeloprasum L., elephant garlic, is a plant that is cultivated and notable for its usage for medical and nutritional purposes. From this point, the present work presents the phenolic profiles of methanol extracts from the flowers, leaves, and bulbs of A. ampeloprasum collected in the wild in Izmir, Turkey, using a liquid chromatography-mass spectrometry technique. Among the 30 quantified phenolic compounds, 3-hydroxybenzoic acid was the major component in A. ampeloprasum from Cambel (AA2) with a value of 3505 +/- 12.8 mu g/g extract, while the bioactive flavonols, quercetin (98.37 +/- 1.75 mu g/g extract), and kaempferol (173.20 +/- 1.3 mu g/g extract) were notable molecules in samples of A. ampeloprasum from Armutlu (AA1). Additionally, antioxidant activity by ferric-reducing antioxidant power and cupric ion-reducing assays, and the total phenolic, and flavonoid content were determined for all extracts. Finally, the samples' capacity to inhibit the enzymes acetylcholinesterase, butyrylcholinesterase, and tyrosinase was evaluated using a 96-well microplate reader, the flowers of both samples generally had stronger inhibitory activity. Furthermore, the relationship between enzyme inhibition potency and the main phenolic components was expounded by molecular modeling studies. Consequently, these experiments were achieved to investigate the prospective usage of A. ampeloprasum both in pharmaceutical and nutraceutical fields. Scientific and Technical Research Council of Turkey (TUBITAK) [217S341]; Pharmaceutical Science Research Centre (FABAL) in Ege University Acknowledgments We thank the Scientific and Technical Research Council of Turkey (TUBITAK) grant no 217S341 and the Pharmaceutical Science Research Centre (FABAL) in Ege University for their financial and technical sup-port, respectively. |
| Databáze: | OpenAIRE |
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