Occurrence and multilocus genotyping of Giardia duodenalis in captive non-human primates from 12 zoos in China

Autor:	Ya Wang, Shumin Yu, Haifeng Liu, Yi Geng, Changliang He, Yufei Wang, Zhihua Ren, Liuhong Shen, Xueping Zhang, Hualin Fu, Xiaobin Gu, Xiaoping Ma, Guangneng Peng, Junliang Deng, Ziyao Zhou, Zhijun Zhong, Dan Jiaming, Cao Suizhong, Yanchun Hu, Xinting Lan, Liqin Wang, Zhicai Zuo
Jazyk:	angličtina
Rok vydání:	2020
Předmět:	0301 basic medicine Giardiasis Range (biology) Protozoan Proteins Monkeys Wildlife Feces 0302 clinical medicine Genotype Clade Phylogeny Mammals Protozoans Multidisciplinary Phylogenetic tree Giardia Eukaryota Vertebrates Medicine Macaque Research Article Primates Lemurs Genotyping China Science Prosimians Animal Types 030231 tropical medicine Zoology Biology Research and Analysis Methods 03 medical and health sciences Phylogenetics Genetic variation Old World monkeys Genetics Animals Molecular Biology Techniques Molecular Biology Organisms Biology and Life Sciences biology.organism_classification Parasitic Protozoans 030104 developmental biology Genetic Loci Amniotes Animals Zoo Giardia lamblia
Zdroj:	PLoS ONE PLoS ONE, Vol 15, Iss 2, p e0228673 (2020)
ISSN:	1932-6203
Popis:	Giardia duodenalis is a common enteric protozoan that infects a range of hosts including humans and other mammals. Multilocus genotyping of G. duodenalis in captive non-human primates (NHPs) from zoos in China is limited. In this study, we evaluated 302 NHP fecal samples collected from 32 different NHP species. The primates were from 12 zoos distributed across eight provinces and two municipalities (Chongqing and Beijing) of China. The overall infection rate was 8.3% (25/302). The six G. duodenalis-positive zoos and their infection rates were: Suzhou Zoo (40.0%, 4/10), Yangzhou Zoo (22.2%, 2/9), Dalian Zoo (16.7%, 4/24), Chengdu Zoo (12.8%, 6/47), Guiyang Forest Wildlife Zoo (12.1%, 7/58), and Changsha Zoo (4.7%, 2/43). Molecular analysis of three loci, beta-giardin (bg), triose phosphate isomerase (tpi), and glutamate dehydrogenase (gdh), showed high genetic heterogeneity, and seven novel subtypes (BIII-1, MB10-1, WB8-1, B14-1, MB9-1, DN7-1, and BIV-1) were detected within assemblage B. Additional analysis revealed 12 different assemblage B multilocus genotypes (MLGs), one known MLG and 11 novel MLGs. Based on phylogenetic analysis, 12 assemblage B MLGs formed two main clades, MLG-SW (10-12, 18) and MLG-SW (13, 14, 16, 17), the other four MLG-SW (15, 19, 20, 21) were scattered throughout the phylogenetic tree in this study. Using multilocus genotyping, this study expands our understanding of the occurrence of Giardia infection and genetic variation in Giardia in captive non-human primates from zoos in China.
Databáze:	OpenAIRE
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