A comparison of precipitation and filtration-based SARS-CoV-2 recovery methods and the influence of temperature, turbidity, and surfactant load in urban wastewater
| Autor: | Cameron Pellett, Jonathan Porter, Nicholas P. Evens, Jessica L. Kevill, Davey Jones, Irene Bassano, Shelagh K. Malham, Steve Paterson, Mathew R. Brown, Jonathan Warren, Andrew C. Singer, Hubert Denise, Kata Farkas, James E. McDonald |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Environmental Engineering
viruses Ultrafiltration Wastewater Article law.invention chemistry.chemical_compound Surface-Active Agents Pulmonary surfactant law PEG ratio Environmental Chemistry Humans Ammonium Turbidity Waste Management and Disposal Filtration Suspended solids SARS-CoV-2 Temperature COVID-19 qRT-PCR Pulp and paper industry Pollution Faecal indicator virus Biology and Microbiology chemistry Wastewater concentration Health RNA detection |
| Zdroj: | The Science of the Total Environment |
| DOI: | 10.1016/j.scitotenv.2021.151916 |
| Popis: | Wastewater-based epidemiology (WBE) has become a complimentary surveillance tool during the SARS-CoV-2 pandemic. Viral concentration methods from wastewater are still being optimised and compared, whilst viral recovery under different wastewater characteristics and storage temperatures remains poorly understood. Using urban wastewater samples, we tested three viral concentration methods; polyethylene glycol precipitation (PEG), ammonium sulphate precipitation (AS), and CP select™ InnovaPrep® (IP) ultrafiltration. We found no major difference in SARS-CoV-2 and faecal indicator virus (crAssphage) recovery from wastewater samples (n = 46) using these methods, PEG slightly (albeit non-significantly), outperformed AS and IP for SARS-CoV-2 detection, as a higher genome copies per litre (gc/l) was recorded for a larger proportion of samples. Next generation sequencing of 8 paired samples revealed non-significant differences in the quality of data between AS and IP, though IP data quality was slightly better and less variable. A controlled experiment assessed the impact of wastewater suspended solids (turbidity; 0–400 NTU), surfactant load (0–200 mg/l), and storage temperature (5–20 °C) on viral recovery using the AS and IP methods. SARS-CoV-2 recoveries were >20% with AS and 0.05), whilst surfactant and storage temperature combined were significant negative correlates (p Graphical abstract Unlabelled Image |
| Databáze: | OpenAIRE |
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