TheMAK16 Gene ofEntamoeba histolytica and Its Identification in Isolates from Patients
| Autor: | Víctor Alberto Marevelez-Acosta, Enedina Jiménez-Cardoso, María del Pilar Crisóstomo-Vázquez, Andrés Flores-Luna |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Male
medicine.medical_specialty Adolescent Virulence Factors Genes Protozoan Gene Expression Brief Communication Young Adult Entamoeba histolytica Cricetinae Molecular genetics Complementary DNA parasitic diseases Gene expression medicine Animals Humans Axenic Gene Genetic Association Studies biology Subtraction hybridization biology.organism_classification subtraction hybridization Molecular biology amebic liver abscess Infectious Diseases mak16 gene Suppression subtractive hybridization Liver Abscess Amebic Parasitology |
| Zdroj: | The Korean Journal of Parasitology |
| ISSN: | 1738-0006 |
| DOI: | 10.3347/kjp.2014.52.4.429 |
| Popis: | To identify sequences of Entamoeba histolytica associated with the development of amebic liver abscess (ALA) in hamsters, subtractive hybridization of cDNA from E. histolytica HM-1:IMSS under 2 growth conditions was performed: 1) cultured in axenic medium and 2) isolated from experimental ALA in hamsters. For this procedure, 6 sequences were obtained. Of these sequences, the mak16 gene was selected for amplification in 29 cultures of E. histolytica isolated from the feces of 10 patients with intestinal symptoms and 19 asymptomatic patients. Only 5 of the 10 isolates obtained from symptomatic patients developed ALA and amplified the mak16 gene, whereas the 19 isolates from asymptomatic patients did not amplify the mak16 gene nor did they develop ALA. Based on the results of Fisher's exact test (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|