Determination of the binding capacity and affinity of proteins from human pregnancy plasma for androstenediol
| Autor: | J Blaquier, M Segredecameo |
|---|---|
| Rok vydání: | 1970 |
| Předmět: |
medicine.medical_specialty
Globulin medicine.medical_treatment Clinical Biochemistry Androstenediol Gestational Age Tritium Biochemistry Steroid chemistry.chemical_compound Endocrinology Pregnancy Internal medicine medicine Humans Testosterone Binding site Molecular Biology Pharmacology Binding Sites Estradiol biology Chemistry Organic Chemistry Estradiol binding medicine.disease biology.protein Plasma binding Female Serum Globulins Androstanes Protein Binding |
| Zdroj: | Steroids. 15:219-226 |
| ISSN: | 0039-128X |
| Popis: | Proteins from late pregnancy plasma were able to bind androstenediol (3β,17β-dihydroxyandrost-5-ene). This steroid and testosterone compete for the binding sites, displacing each other with apparently similar affinity. Estradiol-17β had only 89% of the displacing effect of equal amounts of cold androstenediol. 60% of the plasma binding activity was lost after heating at 60°C for 30′. The binding capacity of pregnancy plasma for androstenediol was: 0.93 ug/g of protein, with apparently two different binding components in the system. A purified globulin fraction had a capacity of 1.35 ug/g of protein. The combining affinity for 1% plasma at 4°C was: 3.49 × 10−3 ml/ug± 0.48 × 10−3. For the globulin fraction the value was: 9.25 × 10−3 ml/ug ± 0.31 × 10−3. The evidence suggests that androstenediol is bound by the same protein already described as testosterone and estradiol binding globulin. |
| Databáze: | OpenAIRE |
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