Potential of bacterial culture media in biofabrication of metal nanoparticles and the therapeutic potential of the as-synthesized nanoparticles in conjunction with artemisinin against MDA-MB-231 breast cancer cells
| Autor: | Badrealam Farheen Khan, Swaleha Zubair, Rituraj Konwar, Mohammad Owais, Hamidullah, Sonam Dwivedi |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Silver Physiology Clinical Biochemistry Nanoparticle Metal Nanoparticles Nanotechnology Antineoplastic Agents Breast Neoplasms Silver nanoparticle Cell Line 03 medical and health sciences 0302 clinical medicine Breast cancer Cell Line Tumor medicine Humans Artemisinin Particle Size Metal nanoparticles health care economics and organizations Mda mb 231 Bacteria Chemistry technology industry and agriculture Cell Biology respiratory system medicine.disease Artemisinins Culture Media 030104 developmental biology HEK293 Cells 030220 oncology & carcinogenesis MCF-7 Cells Female Breast cancer cells medicine.drug Biofabrication |
| Zdroj: | Journal of cellular physiology. 234(5) |
| ISSN: | 1097-4652 |
| Popis: | In the recent past, various groups have proposed diverse biocompatible methods for the synthesis of metal nanoparticles (NPs). Besides culture biomass, culture supernatants (CS) are increasingly being explored for the synthesis of NPs; however, with the ever-increasing exploration of various CS in the biofabrication of NPs, it is equally important to explore the potential of various culture media (CMs) in the synthesis of metal NPs. Considering these aspects, in the present investigation, we explore the possible applicability of various CMs in the biofabrication of metal NPs. The synthesis of NPs was primarily followed by UV/VIS spectroscopy, and, thereafter, the NPs were characterized by various physiochemical techniques, including EM, EDX, FT_IR, X-ray diffraction, and DLS measurements, and finally, their anticancer potentialities were investigated against breast cancer. In addition, the NPs were examined in conjunction with artemisinin for therapeutic benefits against aggressive and highly metastatic MDA-MB-231 breast cancer cells. Cumulatively, the results of the present study collated the potentials of various bacterial CMs in the biofabrication of metal NPs and ascertained the efficacy of the as-synthesized silver nanoparticles, especially the combinatorial entity as intriguing breast cancer therapeutics. The data of the present study plausibly assist in advancing the therapeutic applicability of the combinatorial amalgam against aggressive and highly metastatic MDA-MB-231 breast cancer cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text