A new model of white matter injury in neonatal rats with bilateral carotid artery occlusion
| Autor: | Koh Hasegawa, Tadaki Ohmae, Hideyuki Nagai, Tadashi Sawada, Shoji Kawase, Hiroshi Yoshioka, Hisakazu Uehara |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Pathology
medicine.medical_specialty Internal capsule Leukomalacia Periventricular Hippocampus Cerebral palsy Brain Ischemia Lesion White matter Amyloid beta-Protein Precursor Mice Thalamus Glial Fibrillary Acidic Protein medicine Animals Humans Rats Wistar Molecular Biology Cerebral Cortex Neurons Periventricular leukomalacia Cerebral infarction business.industry General Neuroscience Pyramidal Cells Infant Newborn Brain medicine.disease Rats Disease Models Animal medicine.anatomical_structure Carotid Arteries Cerebral blood flow Animals Newborn Cerebral cortex Regional Blood Flow Astrocytes Cerebrovascular Circulation Neurology (clinical) medicine.symptom business Developmental Biology |
| Zdroj: | Brain research. 837(1-2) |
| ISSN: | 0006-8993 |
| Popis: | Periventricular leukomalacia is an important cause of cerebral palsy and characterized by cysts and coagulation necrosis in the periventricular white matter. Since no model of periventricular leukomalacia has been established in small animals, it is expected to establish a new model of white matter injury in immature rodents. Bilateral carotid arteries were occluded in neonatal rats at 5 days of age, and the brain neuropathologically examined at 7 days of age. Among 22 brains histologically examined, 20 (90.9%) had white matter changes including coagulation necrosis and cystic lesions in and around the internal capsule, while only two had small cerebral infarction and five showed some ischemic neurons in the cerebral cortex. Cerebral blood flow (CBF) decreased to about 25% of controls in the subcortical white matter in the animals with bilateral carotid artery occlusion (BCAO). Amyloid precursor protein (APP) immunohistochemistry demonstrated various APP-immunoreactive axonal profiles in the internal capsule and the subcortical white matter, and stronger expression of APP in pyramidal neurons in the cerebral cortex of BCAO brains. These results indicated that the white matter is more vulnerable than the cerebral cortex in 5-day-old rats when CBF decreases to about 25% and suggested that this model is useful for investigating the white matter changes induced by cerebral hypoperfusion in the neonatal brain, since previous models of hypoxic-ischemic brain injury in neonatal mice and rats revealed preferential susceptibility of the gray matter. It was also indicated that APP is a sensitive marker for mild axonal disruption in the white matter of the immature brain. |
| Databáze: | OpenAIRE |
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