Extent of DNA damage in density-separated trout erythrocytes assessed by the 'comet' assay
| Autor: | Milena Villarini, Donatella Fedeli, Giuseppina Scassellati-Sforzolini, Giancarlo Falcioni, Massimo Moretti, Anna Maria Santroni |
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| Rok vydání: | 1998 |
| Předmět: |
Aging
Erythrocytes DNA damage Alkali labile site Trout Health Toxicology and Mutagenesis DNA Fragmentation Trout erythrocyte DNA condensation medicine.disease_cause chemistry.chemical_compound Genetics medicine Animals Molecular Biology DNA strand break biology Comet test Oxidative stress Mutagenicity Tests biology.organism_classification Comet assay Red blood cell medicine.anatomical_structure chemistry Biochemistry Biophysics Rainbow trout DNA DNA Damage |
| Zdroj: | Mutation research. 397(2) |
| ISSN: | 0027-5107 |
| Popis: | The `comet' assay is being increasingly employed for evaluating DNA damage in biological systems. Using this technique, we examined DNA damage in whole and in density-separated trout erythrocytes. Results clearly show that all the three considered parameters (tail length, tail intensity and tail moment) increased with the density of the fractions, possibly reflecting different degrees of DNA damage. Probably, this behaviour is due to different periods of exposure of the density fractions to the hazard of active oxygen radicals; older cells have been exposed to oxidative stress for a longer time. |
| Databáze: | OpenAIRE |
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