Treatment for chemical burning using liquid crystalline nanoparticles as an ophthalmic delivery system for pirfenidone
| Autor: | Ângelo Malachias, Flavia Rodrigues da Silva, Rummenigge Oliveira Silva, Armando Silva-Cunha, Carolina Nunes da Silva, Bruna Lopes da Costa, Mayara Brandão de Paiva, Adriano Antunes de Souza Araújo, Lays Fernanda Nunes Dourado, Paula Santos Nunes |
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| Rok vydání: | 2019 |
| Předmět: |
Pyridones
Anti-Inflammatory Agents Pharmaceutical Science Nanoparticle Administration Ophthalmic Chick Embryo 02 engineering and technology 030226 pharmacology & pharmacy Chorioallantoic Membrane Cornea 03 medical and health sciences Drug Delivery Systems 0302 clinical medicine Drug Stability Burns Chemical medicine Zeta potential Animals Particle Size Peroxidase Analgesics Polarized light microscopy Chemistry Pirfenidone 021001 nanoscience & nanotechnology Liquid Crystals Drug Liberation Eye Burns medicine.anatomical_structure Transmission electron microscopy Drug delivery Biophysics Nanoparticles Female Rabbits Particle size 0210 nano-technology medicine.drug |
| Zdroj: | International Journal of Pharmaceutics. 568:118466 |
| ISSN: | 0378-5173 |
| Popis: | Some recent studies have shown that pirfenidone (PFD) has favorable results in the healing process of the cornea. However, PFD in solution exhibits short half-life after topical application, and in this context, a liquid crystal nanoparticle system containing PFD (PFD-LCNPs) was developed. The nanoparticles were characterized by transmission electron microscopy, atomic force microscopy, small angle X-ray diffraction and polarized light microscopy. The PFD-LCNPs had particle size and zeta potential of 247.3 nm and −33.60 mV (stores at 4 °C), respectively, and 257.5 nm and −46.00 mV (stored at 25 °C), respectively. The pH of the formulation was 6.9 and the encapsulation efficiency was 35.9%. The in vitro release profiles indicated that PFD sustained release from PFD-LCNPs for up to 12 h. In vitro study of ocular irritation (HET-CAM test) concluded that components of the formulation are well tolerated for ocular administration. Corneal re-epithelialization time after chemical burning was significantly reduced in rabbits treated with PFD-loaded LCNPs when compared to the group treated with a vehicle. In addition, the anti-inflammatory action of pirfenidone was observed by reducing myeloperoxidase activity (MPO) and inflammatory cells in the histology of the tissues of animals treated with PFD-LCNPs. These findings indicated that the PFD-LCNPs might have the potential for effective ocular drug delivery. |
| Databáze: | OpenAIRE |
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