Lack of specific correlation of the deoxycytidine triphosphate pool level with rate of DNA synthesis
Autor: | R.A. Walters, Robert L. Ratliff |
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Rok vydání: | 1975 |
Předmět: |
DNA Replication
Deoxyribonucleoside triphosphate DNA synthesis Deoxycytidine triphosphate Deoxyribonucleotides Cytidine transport Cytidine Cytosine Nucleotides Biology Deoxycytidine Deoxyuridine Biochemistry Genetics and Molecular Biology (miscellaneous) Molecular biology Cell Line chemistry.chemical_compound chemistry Biochemistry heterocyclic compounds Thymidine Nucleotide salvage |
Zdroj: | Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis. 414:221-230 |
ISSN: | 0005-2787 |
DOI: | 10.1016/0005-2787(75)90161-6 |
Popis: | The levels of the four deoxyribonucleoside triphosphate pools and the distribution of cells in the various phases of the cell cycle have been examined in Chinese hamster cells as thymidine, present as a regular constituent in the growth medium, was removed in stages. The results indicate that: 1. 1. Duration of the DNA synthetic phase was lengthened when thymidine was removed from the growth medium. 2. 2. Temporally correlated with lengthening of the DNA synthetic phase upon thymidine removal was a 7-fold increase in level of the dCTP pool, reduction in the dTTP and dGTP pools, and little or no change in dATP pool. 3. 3. Radioactive labeling procedures indicated that expansion of the dCTP pool could be completely accounted for by increased ribonucleotide reductase activity and that the dTTP pool switched from a largely exogenous thymidine source to endogenous dTTP synthesis as the extracellular thymidine concentration was reduced. 4. 4. Deoxyuridine and thymidine were apparently transported by the same system in Chinese hamster cells, while deoxycytidine was transported by a different system. Although deoxycytidine transport was unaffected by thymidine, phosphorylation of intracellular deoxycytidine compounds to the triphosphate level was stimulated by thymidine. Cytidine transport was not significantly affected by thymidine. |
Databáze: | OpenAIRE |
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