Guiding pancreatic beta cells to target electrodes in a whole-cell biosensor for diabetes
| Autor: | Fanny Lebreton, Sylvie Renaud, Romain Perrier, Stéphane Arbault, Jochen Lang, Antoine Pirog, Alexander Kuhn, Aleksandar Karajić, Matthieu Raoux, Julien Gaitan, Eileen Pedraza |
|---|---|
| Přispěvatelé: | Chimie et Biologie des Membranes et des Nanoobjets (CBMN), Université de Bordeaux (UB)-École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut des Sciences Moléculaires (ISM), Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de l'intégration, du matériau au système (IMS), Université Sciences et Technologies - Bordeaux 1-Institut Polytechnique de Bordeaux-Centre National de la Recherche Scientifique (CNRS), ANR-13-PRTS-0017,ISLET CHIP,ISLET CHIP: Contrôle de Qualité d'Îlots pour la Greffe(2013), ANR-10-LABX-0042,AMADEus,Advanced Materials by Design(2010), ANR-10-IDEX-0003,IDEX BORDEAUX,Initiative d'excellence de l'Université de Bordeaux(2010) |
| Rok vydání: | 2015 |
| Předmět: |
Male
Cell type Insulin/metabolism Cell Biomedical Engineering Bioengineering Biosensing Techniques 02 engineering and technology Biology 01 natural sciences Biochemistry Mice Biosensing Techniques/methods Insulin-Secreting Cells Insulin Secretion Diabetes Mellitus medicine Animals Humans Insulin Diabetes Mellitus/diagnosis [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics Insulin secretion Tissue Array Analysis/methods Electrodes Cells Cultured Pancreatic islets 010401 analytical chemistry Electric Conductivity General Chemistry 021001 nanoscience & nanotechnology Electrophysiological Phenomena 0104 chemical sciences Mice Inbred C57BL Electrophysiology Electrophoresis medicine.anatomical_structure Tissue Array Analysis Electrode Insulin-Secreting Cells/cytology/metabolism Female 0210 nano-technology Biosensor Biomedical engineering |
| Zdroj: | Lab on a Chip Lab on a Chip, Royal Society of Chemistry, 2015, 15, ⟨10.1039/C5LC00616C⟩ Lab on a Chip, Vol. 15, No 19 (2015) pp. 3880-3890 |
| ISSN: | 1473-0189 1473-0197 |
| DOI: | 10.1039/c5lc00616c |
| Popis: | International audience; We are developing a cell-based bioelectronic glucose sensor that exploits the multi-parametric sensing ability of pancreatic islet cells for the treatment of diabetes. These cells sense changes in the concentration of glucose and physiological hormones and immediately react by generating electrical signals. In our sensor, signals from multiple cells are recorded as field potentials by a micro-electrode array (MEA). Thus, cell response to various factors can be assessed rapidly and with high throughput. However, signal quality and consequently overall sensor performance rely critically on close cell–electrode proximity. Therefore, we present here a non-invasive method of further exploiting the electrical properties of these cells to guide them towards multiple micro-electrodes via electrophoresis. Parameters were optimized by measuring the cell's zeta potential and modeling the electric field distribution. Clonal and primary mouse or human β-cells migrated directly to target electrodes during the application of a 1 V potential between MEA electrodes for 3 minutes. The morphology, insulin secretion, and electrophysiological characteristics were not altered compared to controls. Thus, cell manipulation on standard MEAs was achieved without intro- ducing any external components and while maintaining the performance of the biosensor. Since the analy- sis of the cells' electrical activity was performed in real time via on-chip recording and processing, this work demonstrates that our biosensor is operational from the first step of electrically guiding cells to the final step of automatic recognition. Our favorable results with pancreatic islets, which are highly sensitive and fragile cells, are encouraging for the extension of this technique to other cell types and microarray devices. |
| Databáze: | OpenAIRE |
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