Stable Isotope-Resolved Metabolomics Studies on Corticosteroid-Induced PC12 Cells: A Strategy for Evaluating Glucose Catabolism in an in Vitro Model of Depression
| Autor: | Xue-Mei Qin, Linghu Ting, Yao Gao, Shao-Bo Liu, Jun-Sheng Tian, Yun-Hao Zhao, Wen-Ze Wu |
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| Rok vydání: | 2021 |
| Předmět: |
Male
medicine.medical_specialty Nephrotic Syndrome Citric Acid Cycle Pentose phosphate pathway Biochemistry PC12 Cells Isotopes Adrenal Cortex Hormones Internal medicine Pyruvic Acid medicine Animals Humans Metabolomics Purine metabolism business.industry Depression General Chemistry Metabolism Rats Citric acid cycle Metabolic pathway Endocrinology Glucose Gluconeogenesis Antidepressant Female business Flux (metabolism) |
| Zdroj: | Journal of proteome research. 21(3) |
| ISSN: | 1535-3907 |
| Popis: | Depression is a common psychopathological state or mood disorder syndrome. The serious risks to human life and the inadequacy of the existing antidepressant drugs have driven us to understand the pathogenesis of depression from a new perspective. Our research group has found disturbances in glucose catabolism in both depression and nephrotic syndrome. What are the specific metabolic pathways and specificities of glucose catabolism disorders caused by depression? To address the above scientific questions, we creatively combined traditional metabolomics technology with stable isotope-resolved metabolomics to research the glucose catabolism of the corticosterone-induced PC12 cell damage model and the adriamycin-induced glomerular podocyte damage model. The results showed an increased flux of pyruvate metabolism in depression. The increased flux of pyruvate metabolism led to an activation of gluconeogenesis in depression. The disturbed upstream metabolism of succinate caused the tricarboxylic acid cycle (TCA cycle) to be blocked in depression. In addition, there were metabolic disturbances in the purine metabolism and pentose phosphate pathways in depression. Compared with nephrotic syndrome, pyruvate metabolism, the TCA cycle, and gluconeogenesis metabolism in depression were specific. The metabolic pathways researched above are likely to be important targets for the efficacy of antidepressants. |
| Databáze: | OpenAIRE |
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