Microtubules in PMNs from Patients with Familial Mediterranean Fever
| Autor: | Arthur D. Schwabe, L. Wilson, M. Bar-Eli, Mary C. Territo, Robert S. Peters |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Adult
Male Adolescent Neutrophils Familial Mediterranean fever In Vitro Techniques Microtubules Colchicine treatment chemistry.chemical_compound Tubulin Microtubule In vivo Concanavalin A Humans Medicine Colchicine Cap formation biology business.industry General Medicine Middle Aged medicine.disease Molecular biology In vitro Familial Mediterranean Fever chemistry Immunology biology.protein Female business Protein Binding |
| Zdroj: | The American Journal of the Medical Sciences. 284:2-7 |
| ISSN: | 0002-9629 |
| DOI: | 10.1097/00000441-198209000-00001 |
| Popis: | Polymorphonuclear (PMN) cells derived from patients with Familial Mediterranean Fever (FMF) were evaluated in vitro for the function and concentration of their microtubules. Using the time-decay colchicine binding assay to determine the tubulin concentration in PMN cells, no difference was found in PMN cells derived from FMF patients in comparison with those from normal healthy subjects. Colchicine treatment had no effect on the detectable tubulin concentration in the cells. The mobility of fluorescent con A(F-con A)-receptor complexes on PMN membranes was used to test the function of the microtubules. PMNs from untreated FMF patients showed the same pattern of con A cap formation as seen in normal cells. PMNs derived from colchicine treated patients, however, showed 22-32% spontaneous cap formation. These cells also showed 10-30% more capping in comparison with normal or untreated FMF cells, for any given in vitro colchicine concentration, suggesting that at therapeutic doses, the colchicine does accumulate in the PMNs in vivo. We were unable to demonstrate a microtubule defect in the neutrophils from FMF patients in these studies. |
| Databáze: | OpenAIRE |
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