Time-resolved fluorescence immunoassay for urine retinol-binding protein is more sensitive than polyclonal and monoclonal assays
| Autor: | Mark Dockrell, Ekramun Nabi, Rashim Salota, Steve Hyer, Marta Lapsley, Simon Packer |
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| Rok vydání: | 2021 |
| Předmět: |
Adult
Male 030213 general clinical medicine Fluoroimmunoassay Clinical Biochemistry 030232 urology & nephrology Enzyme-Linked Immunosorbent Assay Urine 03 medical and health sciences 0302 clinical medicine Albuminuria Humans Aged Chromatography biology Chemistry Antibodies Monoclonal General Medicine Middle Aged Fluorescence immunoassay Time resolved fluorescence immunoassay Retinol binding protein Tubular proteinuria Polyclonal antibodies Monoclonal biology.protein Female Kidney Diseases Retinol-Binding Proteins Plasma |
| Zdroj: | Annals of Clinical Biochemistry: International Journal of Laboratory Medicine. 58:505-519 |
| ISSN: | 1758-1001 0004-5632 |
| DOI: | 10.1177/00045632211020034 |
| Popis: | Background Retinol-binding protein4 (RBP) assays using polyclonal antibodies (pRBP) have major problems of non-linearity of dilution and a very small useable dynamic range. Our objective was to develop a specific assay with a wider dynamic range to detect tubular proteinuria. Methods mRBP (monoclonal capture and second antibody with colorimetric detection) and fluoroimmunoassays for RBP (fRBP) (polyclonal capture and monoclonal second antibody with fluorescence detection) were developed and compared with pRBP. Four hundred and eighty-eight patient samples were collected; 290 samples were analysed by mRBP and 198 samples with fRBP and compared with pRBP. Results mRBP assay has the advantages of better linearity on dilution and wider analytical range over pRBP. It is limited by poor signal in the patients with albuminuria and glomerular proteinuria and inferior discrimination between patient groups. fRBP had an intra-assay and inter-assay CV of Conclusions The combination assay with fluorescence detection (fRBP) proved more discriminatory than a purely monoclonal system especially in patients with significant proteinuria and has advantages of better linearity on dilution and wider analytical range than the existing pRBP assay and compared extremely well with pRBP. |
| Databáze: | OpenAIRE |
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